文献类型：期刊文献

英文题名：A Multi-Channel Activatable Nanoprobe for Simultaneous Subcellular Profiling of Multiple Cancer Biomarkers

作者：Lv, Jian[1]; Chang, Shuai[1]; Wang, Xiao-Yuan[1]; Chen, Hua-Ying[1]; Chen, Zhen-Chi[1]; Chen, Bin-Bin[1]; Zhou, Xin-Yue[1]; Qian, Ruo-Can[1]; Li, Da-Wei[1]

机构：[1] Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center Frontiers Science Center for Materiobiology & Dynamic Chemistry School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220453629)

语种：英文

外文关键词：Biomarkers - Cancer cells - Clinical research - Cytology - Diagnosis - Diseases - Fluorescence imaging - Gold compounds - Gold nanoparticles - Lanthanum compounds - Raman spectroscopy - RNA

摘要：The aberrant change of the expression profile of multiple cancer biomarkers is closely related to tumor progression and therapeutic effect. So far, simultaneous imaging of multiple cancer biomarkers has remained a great challenge due to their low abundance in living cells and limitations of existing imaging strategies. Here, we report a multi-modal imaging strategy to detect the correlated expression of multiple cancer biomarkers, MUC1, microRNA-21 (miRNA-21) and ROS in living cells, based on a porous covalent organic framework (COF) wrapped gold nanoparticles (AuNPs) core-shell nanoprobe. The nanoprobe is functionalized with Cy5-labeled MUC1 aptamer, a ROS-responsive molecule (2-mercaptohydroquinone, 2-MHQ), and a miRNA-21-response hairpin DNA tagged by FITC as the reporters for different biomarkers. The target-specific recognition can induce the orthogonal molecular change of these reporters, producing fluorescence and Raman signals for imaging the expression profiles of membrane MUC1 (red fluorescence channel), intracellular miRNA-21 (green fluorescence channel), and intracellular ROS (SERS channel). Using HeLa cells, we demonstrate the successful simultaneous detection of MUC1, miRNA-21 and ROS in single cancer cells. We further demonstrate the capability of the nanoprobe for the imaging of subcellular expression changes of MUC1, miRNA-21 and ROS in response to the treatment of ROS stimulants, as the cooperative expression of these biomarkers is observed, along with the activation of NF-κB pathway. Our research provides a robust platform for the imaging of multiple cancer biomarkers, with broad potential applications in cancer clinical diagnosis and drug discovery. ? 2022, The Authors. All rights reserved.