文献类型：期刊文献

英文题名：Identification of carbohydrates as the major carbon sink of the marine microalga Isochrysis zhangjiangensis (Haptophyta) and optimization of its productivity by nitrogen manipulation

作者：Wang, Hai-Tao[1,2];Yao, Chang-Hong[1];Ai, Jiang-Ning[1];Cao, Xu-Peng[1];Xue, Song[1];Wang, Wei-liang[3]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：171

期号：1

起止页码：298

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20144400136231);WOS:【SCI-EXPANDED(收录号:WOS:000343091700043)】；

基金：This work was supported by National Key Basic Research Program of China "973 Program" (2011CBA00803), National High Technology Research and Development Program "863" (2012AA052101 and 2014AA022004), Liaoning Provincial Natural Science Foundation (2012010263), the Hundred Talent Program of the Chinese Academy of Sciences (No. A1097) and Knowledge Innovation Programs of Dalian Institute of Chemical Physics, CAS (K2010A3).

语种：英文

外文关键词：Isochrysis zhangjiangensis; Carbohydrate production; Nitrogen limitation; Microalga carbohydrate

摘要：Microalgae represent a potential feedstock for biofuel production. During cultivation under nitrogen-depleted conditions, carbohydrates, rather than neutral lipids, were the major carbon sink of the marine microalga Isochrysis zhangjiangensis (Haptophyta). Carbohydrates reached maximum levels of 21.2 pg cell(-1) on day 5, which was an increase of more than 7-fold from day 1, while neutral lipids simultaneously increased 1.9-fold from 4.0 to 7.6 pg cell(-1) during the ten-day nitrogen-depleted cultivation. The carbohydrate productivity of I. zhangjiangensis was improved by optimization of the nitrate supply mode. The maximum carbohydrate concentration was 0.95 g L-1 under batch cultivation, with an initial nitrogen concentration of 31.0 mg L-1, which was 2.4-fold greater than that achieved under nitrogen-depleted conditions. High performance liquid chromatography (HPLC) analysis showed that the accumulated carbohydrate in I. zhangjiangensis was composed of glucose. These results show that I. zhangjiangensis represents an ideal carbohydrate-enriched bioresource for biofuel production. (C) 2014 Elsevier Ltd. All rights reserved.