文献类型：期刊文献

英文题名：Development of a Process for Separation of Mogroside V from Siraitia grosvenorii by Macroporous Resins

作者：Zhang, Min[2];Yang, Huihua[1];Zhang, Hongyang[3];Wang, Yuerong[3];Hu, Ping[3]

机构：[1]Guilin Univ Elect Technol, Coll Comp Sci & Control, Guilin 541004, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：16

期号：9

起止页码：7288

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000295211000009)】；

基金：The authors acknowledge financial support for this work from the National Natural Science Foundation of China (Number 30860381, 21006023) and Shanghai-Unilever Research and Development Fund (Number 08520750300).

语种：英文

外文关键词：mogrosides V; Siraitia grosvenorii; macroporous resins; separation; enrichment

摘要：A separation method was developed for the preparative separation and enrichment of the non-caloric sweetener mogroside V from Siraitia grosvenorii. The adsorption properties of six macroporous resins were evaluated. Results showed that HZ 806 resin offered the best adsorption and desorption capacities. Based on the adsorption experiments on HZ 806, the adsorption data were found to fit the Freundlich model well. The pseudo-second-order kinetic model showed the highest correlation with the experimental results. Separation was performed with deionized water and 40% aqueous ethanol solution as mobile phases. In a typical run, 100 g of herb was processed and 3.38 g of mogroside V with a purity of 10.7% was harvested. This separation method provided a 15.1-fold increase in the purification factor from 0.5% to 10.7%. The present study showed that HZ 806 resins were effective for the separation and enrichment of mogroside V from S. grosvenorii.