文献类型：期刊文献

英文题名：2-Amino-3′-dialkylaminobiphenyl-based fluorescent intracellular probes for nitric oxide surrogate N2O3

作者：Escamilla, P. Rogelio[3];Shen, Yanming[1];Zhang, Quanjuan[1];Hernandez, Derek S.[3];Howard, Cecil J.[3];Qian, Xuhong[1,2];Filonov, Daria Y.[4];Kinev, Alexander V.[4];Shear, Jason B.[3];Anslyn, Eric V.[3];Yang, Youjun[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Meilong Rd 130, Shanghai 200237, Peoples R China;[3]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA;[4]Creative Scientist Inc, Durham, NC USA

年份：2020

卷号：11

期号：5

起止页码：1394

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20200708164185);WOS:【SCI-EXPANDED(收录号:WOS:000515700500021)】；

基金：JBS acknowledges support from Welch Foundation Grant F-1331 for these studies. AVK is a recipient of SBIR Phase I grant 1R43ES027716 from the National Institute of Environmental Health Sciences. EVA acknowledges.nancial support from HHMI Professorship Program, and the Welch Regents Chair (F-0046). YY acknowledges the National Natural Science Foundation of China (No. 21572061, 21602181, and 21822805).

语种：英文

外文关键词：Cell culture - Fluorophores - Probes

摘要：Fluorescent probes for nitric oxide (NO), or more frequently for its oxidized surrogate dinitrogen trioxide (N2O3), have enabled scientists to study the contributions of this signaling molecule to many physiological processes. Seeking to improve upon limitations of other probes, we have developed a family of fluorescent probes based on a 2-amino-3 '-dialkylaminobiphenyl core. This core condenses with N2O3 to form benzo[c]cinnoline structures, incorporating the analyte into the newly formed fluorophore, which results in product fluorescence with virtually no background contribution from the initial probe. We varied the substituents in the core in order to optimize both the reactivity of the probes with N2O3 and their cinnoline products' fluorescence wavelengths and brightness. The top candidates were then applied to cultured cells to verify that they could respond to NO within cellular milieus, and the top performer, NO530, was compared with a "gold standard" commercial probe, DAF-FM, in a macrophage-derived cell line, RAW 264.7, stimulated to produce NO. NO530 demonstrated similar or better sensitivity and higher selectivity for NO than DAF, making it an attractive potential alternative for NO tracking in various applications.