文献类型：期刊文献

英文题名：Antibacterial activity and mechanism of the sesquiterpene 8-cadinene against Listeria monocytogenes

作者：Qin, Rong[1];Yang, Shengping[1];Fu, Bin[1];Chen, Yang[1];Zhou, Mengzhou[1];Qi, Yonggang[1];Xu, Ning[1];Wu, Qian[1];Hua, Qiang[2];Wu, Yongkang[3];Liu, Zhijie[1]

机构：[1]Hubei Univ Technol, Cooperat Innovat Ctr Ind Fermentat, Natl Ctr Cellular Regulat & Mol Pharmaceut 111, Key Lab Fermentat Engn,Minist Educ & Hubei Prov, Wuhan 430068, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Hubei Juhui Agr Co Ltd, Jingmen 431821, Peoples R China

年份：2024

卷号：203

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001260704700001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (22007031) , Key Research and Development Program of Hubei Province (2023BBB010) , Hubei Provincial Natural Science Foundation of China (2023AFB927) , Major Science and Technology Innovation Plan Project of Jingmen City (2024ZDYF15) , and Collaborative Grant -in -Aid of the HBUT National "111" Center for Cellular Regulation and Molecular Pharmaceutics (XBTK-2022012) .

语种：英文

外文关键词：8-cadinene; Listeria monocytogenes; Antibacterial activity; Antimicrobial mechanism; Transcriptome analysis

摘要：Listeria monocytogenes is a food-borne pathogen that seriously threatens human health. We analyzed the antibacterial activity and mechanism of 8-cadinene against L. monocytogenes. The effect of 8-cadinene on L. monocytogenes gene expression was investigated using RNA sequencing. 8-Cadinene inhibited L. monocytogenes growth at a minimum inhibitory concentration of 5 mu L/mL and a minimum bactericidal concentration of 10 mu L/ mL. In addition, 8-cadinene suppressed biofilm formation, altered cell morphology, and damaged the cell membrane of L. monocytogenes, enhancing membrane permeability and affecting the membrane potential. Of note, "plasma membrane" and "membrane" were the most significantly enriched Gene Ontology terms. Sixteen differentially expressed genes were strongly correlated with biofilm formation, protein and nucleic acid leakage, and membrane potential. The growth of L. monocytogenes in milk was effectively controlled with 10 mu L/mL 8-cadinene, especially at 4 degrees C. In conclusion, treatment with 8-cadinene could be a potential strategy for controlling the contamination of foods by L. monocytogenes.