文献类型：期刊文献

中文题名：K^+/Na^+浓度比对罗汉果悬浮细胞生长和甜苷V合成的影响

英文题名：Physiologically affects of ratio of K^+ to Na^+ on cell growth and mogrosides V biosynthesis in Siraitia grosvenorii cells cultivated in suspension culture

作者：陈雨霞[1];王泽建[1];肖慈英[1];庄英萍[1,2];宋云飞[3];郭美锦[1,2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海生物制造技术协同创新中心,上海200237;[3]桂林莱茵生物科技股份有限公司,广西桂林541199

年份：2018

卷号：54

期号：5

起止页码：812

中文期刊名：植物生理学报

外文期刊名：Plant Physiology Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家科技重大专项(2017ZX07402003)~~

语种：中文

中文关键词：罗汉果;悬浮培养;K^+/Na^+浓度比;甜苷V生物合成

外文关键词：Siraitia grosvenorii;suspension culture;K^＋/Na^＋ concentration ratio;mogrosides V biosynthesis

摘要：本文研究了不同K^+/Na^+浓度比对罗汉果悬浮细胞生长和甜苷V合成的影响。结果表明,合适的K^+/Na^+浓度比促进细胞生长和甜苷V生物合成。当K^+/Na^+最佳浓度比为15:1时,罗汉果悬浮细胞内K^+浓度、K^+/Na^+、S_((K,Na))值均为最大,此时细胞利用K^+效率最高,细胞生长速率最快。此外,胞外有机酸和胞内氨基酸的变化表明此浓度比例下,细胞可能通过降低TCA循环代谢强度,以及增强EMP途径和MVA途径代谢强度,从而促进萜类化合物前体乙酰Co A向罗汉果甜苷V合成。
The effect of K^＋/Na^＋ratios on the cell growth and mogrosides V production in suspended Siraitia grosvenorii cells culture were investigated in this paper.The results showed that an appropriate ratio of K^＋to Na^＋could significantly improve the cell growth as well as mogrosides V biosynthesis.Under the optimal condition of at 15：1,the intracellular K^＋cell,K^＋/Na^＋ratio and S（K,Na）were observed at their maximum values,simultaneously,the highest K^＋uptaken rate and cell growth rate were also obtained.Moreover,through the extracellular organic acid and amino acid profiles it was inferred that at the level of K^＋/Na^＋ratio of 15：1,the decrease of TCA metabolic flux as well as the increases of EMP metabolic flux and MVA metabolic flux could lead to the precursor acetyl Co A utilization for mogrosides V production.