文献类型：期刊文献

英文题名：A near-infrared fluorescent probe for fast and precise imaging of senescent cells and ovarian cancer cells via tracking β-galactosidase

作者：Pan, Haiting[1];Chai, Xianzhi[2];Zhang, Junji[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn,Key Lab Adv MatJoint Int Res L, Shanghai 200237, Peoples R China;[2]Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, Zhenjiang 212100, Peoples R China

年份：2023

卷号：34

期号：12

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001149364100001)】；

基金：This work is supported by National Natural Science Foundation of China (Nos. 22122803 and 21788102) and the National Natural Science Foundation of Jiangsu Province (No. BK20220644) .

语种：英文

外文关键词：beta-Galactosidase; Near-infrared fluorescent probe; Senescent cell; Precise imaging; Fast response

摘要：Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society. Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associated beta-galactosidase (SA-beta-Gal), which is proved to be closely associated with senescent cells, the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associated beta-Gal (OA-beta-Gal) will interfere with imaging accuracy. Herein, a near-infrared (NIR) hemicyanine based fluorescent probe HCyXA-beta Gal was designed for light-up imaging of live cells containing beta-Gal. With the organelle-targeting morpholinyl and positive charge moieties, HCyXA- beta Gal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells. Furthermore, inspired by the fast response performance, fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal: time-dependent intensity. (c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.