文献类型：期刊文献

英文题名：Construction of developmentally inspired periosteum-like tissue for bone regeneration

作者：Dai, Kai[1,2];Deng, Shunshu[1,2];Yu, Yuanman[1,2];Zhu, Fuwei[1,2];Wang, Jing[1,2];Liu, Changsheng[2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai, Peoples R China

年份：2022

卷号：10

期号：1

外文期刊名：BONE RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000737063000001)】；

基金：We thank Prof. R. Yue at Tongji University for his kind donation of Lepr-Cre; tdTomato mice and C. Y. MO at Tongji University for her technical support in sample digestion. We thank T. Shen at East China University of Science and Technology (ECUST) for her technical support in the in vivo imaging assay. Our work on laser scanning confocal microscopy imaging was performed at the National Center for Protein Science Shanghai with the aid of F. M. Liu and Y. Yu. Our flow cytometry work was performed at Shanghai Jiao Tong University School of Medicine (SHSMU) Flow Cytometry Core with the aid of R. Fu, X. Zhang, and C. Guo, as well as East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Flow Cytometry Core with the aid of C Wang and Y. L. Xia. This research was supported by the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the National Key R&D Program of China (NO. 2018YFE0201500), the National Natural Science Foundation of China (No. 31870953), the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry (NO. JKVD1211002), and the Weigao Project of Chinese Academy of Sciences [NO. (2020) 005].

语种：英文

摘要：The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor resection. Here, we utilized a bone morphogenetic protein-2 (BMP-2)-loaded scaffold to create periosteum-like tissue (PT) in vivo, mimicking the mesenchymal condensation during native long bone development. We found that BMP-2-induced endochondral ossification plays an indispensable role in the construction of PTs. Moreover, we confirmed that BMP-2-induced PTs exhibit a similar architecture to the periosteum and harbor abundant functional periosteum-like tissue-derived cells (PTDCs), blood vessels, and osteochondral progenitor cells. Interestingly, we found that the addition of chondroitin sulfate (CS), an essential component of the extracellular matrix (ECM), could further increase the abundance and enhance the function of recruited PTDCs from the PTs and finally increase the regenerative capacity of the PTs in autologous transplantation assays, even in old mice. This novel biomimetic strategy for generating PT through in vivo endochondral ossification deserves further clinical translation.