文献类型：期刊文献

英文题名：Analysis of metabolic fluxes for better understanding of mechanisms related to lipid accumulation in oleaginous yeast Trichosporon cutaneum

作者：Liu, Zhijie[1];Gao, Yang[1];Chen, Jun[1];Imanaka, Tadayuki[1,2];Bao, Jie[1];Hua, Qiang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ritsumeikan Univ, Coll Life Sci, Dept Biotechnol, Kusatsu 5258577, Japan

年份：2013

卷号：130

起止页码：144

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000316032300020)】；

基金：This work was financially supported by National Basic Research Program of China (973 Program) (2012CB721101), Fundamental Research Funds for the Central Universities of China (WF0913005), Research Fund for the Doctoral Program of Higher Education of China (20110074110014), and partly supported by National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), and Shanghai Leading Academic Discipline Project (B505). The authors are grateful to the help provided by Professor Siliang Zhang in fermentation and Li Zhou in GC-MS analysis.

语种：英文

外文关键词：Metabolic flux analysis; Trichosporon cutaneum; Biodiesel; Malic enzyme; Citrate pyruvate cycle

摘要：Microbial fermentation for producing biodiesel from lignocellulosic hydrolysates is receiving increasing attention and attempts have been made to screen an oleaginous Trichosporon sp. with high lipid content and a strong tolerance to lignocellulose hydrolysates. In order to better understand mechanisms related to its lipid accumulation, metabolic flux analysis was performed under 5 g L-1 ammonium sulfate (high nitrogen) and/or 0.4 g L-1 ammonium sulfate (low nitrogen) conditions. Cell growth phase and lipid accumulation phase were shown for cells grown under low nitrogen condition. Results of flux distribution demonstrated that NADPH provided by cytosolic malic enzyme and the acetyl-CoA from cytoplasmic citrate by the ATP: citrate lyase were the two primary sources for excess lipid accumulation. Flux data also supported the fact that the citrate pyruvate cycle plays an essential role in the lipid accumulation. The flux information obtained could also motivate new design strategies for oleaginous yeasts for enhanced biodiesel production. (C) 2012 Elsevier Ltd. All rights reserved.