文献类型：期刊文献

英文题名：MAPKpathway-related tyrosine phosphatases regulate development, secondary metabolism and pathogenicity in fungusAspergillus flavus

作者：Yang, Guang[1,2];Cao, Xiaohong[1,2];Ma, Genli[1,2];Qin, Ling[1,2];Wu, Yuanzhen[3];Lin, Jian[1,2];Ye, Peng[1,2];Yuan, Jun[1,2];Wang, Shihua[1,2]

机构：[1]Fujian Agr & Forestry Univ, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Fuzhou 350002, Peoples R China;[2]Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China;[3]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China

年份：2020

卷号：22

期号：12

起止页码：5232

外文期刊名：ENVIRONMENTAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000566515900001)】；

基金：This work was funded by the National Natural Science Foundation of China (No. 31772105), the Natural Science Foundation of Fujian Province of China (No. 2018J07002) and the Scientific Research Foundation of Fujian Agriculture and Forestry University (324-1122yb042). In particular, we would like to thank Dr. Perng Kuang Chang (Southern Regional Research Center, United States Department of Agriculture, New Orleans, USA) and Prof. Yang Liu (Institute of Food Science and Technology, CAAS) for kindly providing the strains. We thank an English editing agency (Meiji, ) and an English-speaking colleague for linguistic assistance during the preparation of this manuscript.

语种：英文

摘要：Mitogen-activated protein kinase (MAPK) cascades are highly conserved in eukaryotic cells and are known to play crucial roles in the regulation of various cellular processes. However, compared with kinase-mediated phosphorylation, dephosphorylation catalysed by phosphatases has not been well characterized in filamentous fungi. In this study, we identified five MAPK pathway-related phosphatases (Msg5, Yvh1, Ptp1, Ptp2 and Oca2) and characterized their functions inAspergillus flavus, which produces aflatoxin B-1(AFB(1)), one of the most toxic and carcinogenic secondary metabolites. These five phosphatases were identified as negative regulators of MAPK (Slt2, Fus3 and Hog1) pathways. Deletion ofMsg5andYvh1resulted in significant defects in conidiation, sclerotia formation, aflatoxin production and crop infection. Additionally, double knockout mutants (Delta Msg5/Delta Ptp1, Delta Msg5/Delta Ptp2and Delta Msg5/Delta Oca2) displayed similar defects to those observed in the Delta Msg5single mutant, indicating that Msg5 plays a major role in the regulation of development and pathogenicity inA.flavus. Importantly, we found that the active site at C439 is essential for the function of the Msg5 phosphatase. Furthermore, the MAP kinase Fus3 was found to be involved in the regulation of development, aflatoxin biosynthesis and pathogenicity, and its conserved phosphorylation residues (Thr and Tyr) were critical for the full range of its functions inA.flavus. Overall, our results reveal that MAPK related tyrosine phosphatases play important roles in the regulation of development, secondary metabolism and pathogenicity inA.flavus, and could be developed as potential targets for preventing damage caused by this fungal pathogen.