文献类型：专利

英文题名：Preparing hydrogel used for preparing artificial skin involves mixing precursor and aqueous collagen solution and then bi-crosslinking mixture through enzyme pre-crosslinking and physical crosslinking

作者：CHEN D;LU T;LU Y;YANG K;YIN R;ZHANG H;ZHANG W

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY;[2]BLACKSHIP TECHNOLOGY SHANGHAI CO LTD

申请号：CN105944150-A

申请日：2016-03-25

公开日：2016-09-21

语种：英文

收录：DERWENT

摘要：NOVELTY - Preparing hydrogel involves mixing the precursor and the aqueous collagen solution and then bi-crosslinking the mixture through enzyme pre-crosslinking and physical crosslinking, where the precursor is an aqueous solution of 5-12 wt.% chitosan, 3-9 wt.% tyrosinase and 3-10 wt.% hyaluronic acid. USE - Method for preparing hydrogel used for preparing artificial skin, which is used for cell growth and encapsulation of cell (claimed). ADVANTAGE - The method enables to prepare hydrogel, that helps preparing artificial skin with mechanical properties similar to human skin, repairs and regenerates skin tissue. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for artificial skin preparation, which involves: (A) preparing a hydrogel and building a cortex using three-dimensional printing technology; (B) carrying out ultrasonic atomization of a glutaraldehyde solution or an N-hydroxysuccinimide solution for crosslinking the skin layer constructed; and (C) repeating the steps to obtain a multi-layer artificial skin.