文献类型：期刊文献

英文题名：Long-term low-dose oxytetracycline potentially leads to neurobehavioural changes

作者：Qiu, Yushu[1];Yu, Kan[1,2,5];Yu, Xiaogang[3,4];Yi, Xiaoping[2];Cai, Kang[1];Li, Huajun[1];Xu, Shanshan[1];Wang, Weiye[3,4];Huang, Lisu[1]

机构：[1]Shanghai Jiao Tong Univ, Dept Infect Dis, Xinhua Childrens Hosp, Xinhua Hosp,Sch Med, Shanghai 200092, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Minist Educ, Xinhua Hosp, Sch Med, Shanghai 200092, Peoples R China;[4]Shanghai Jiao Tong Univ, Shanghai Key Lab Childrens Environm Hlth, Xinhua Hosp, Sch Med, Shanghai 200092, Peoples R China;[5]Fudan Univ, Sch Life Sci, Shanghai 200438, Peoples R China

年份：2021

卷号：223

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000685543500011)】；

基金：This work was supported by the National Natural Science Foundation of China [No. 82073561, 81874265, 81530086] , the Shanghai Munic-ipal Health Commission [No. 2017YQ033] , the Shanghai Municipal Human Resources and Social Security Bureau [No. 2018003] , and the Shanghai Science and Technology Commission [No. 18411966600, 19410740800] .

语种：英文

外文关键词：Antibiotic exposure; Oxytetracycline in the environment; Behaviour change; Neurotransmitter; Thyroid disruption

摘要：Trace levels of oxytetracycline (OTC)-a veterinary antibiotic and feed additive-are widespread in the environment. Studies revealed that OTC potentially impairs thyroid function, which may affect neurobehaviour; however, the impact of exposure to environmental concentrations of OTC on adult neurobehaviour is unknown. In this study, the effects of OTC on zebrafish after 30-day exposure were investigated. The total swimming distance was significantly increased under vibration and light/dark stimulation, while time spent in the white area was prolonged during the black/white preference test, indicating that the zebrafish became bolder and more impulsive under low OTC exposure. Additionally, monoamine neurotransmitter (5-hydroxytryptamine, dopamine, norepinephrine) levels were decreased and gene expression of monoamine oxidase (mao) involved in neurotransmitter metabolism was upregulated at the transcription level after OTC exposure. Because triiodothyronine (T3) levels were enhanced following exposure to OTC, we speculated that T3 may mediate OTC damage to the nervous system. Our simulated molecular docking analysis showed that OTC combined with the sodium iodide cotransporter protein may result in excessive T3 synthesis. We further exposed zebrafish to T3, and they exhibited similar behaviour to the OTC exposure group. In conclusion, environmental OTC may activate monoamine oxidase and enhance the metabolism of monoaminergic neurotransmitters via T3, thereby inducing abnormal neurobehaviour.