文献类型：期刊文献

英文题名：Mechanism of ZnFe 2 O 4 NPs on biohydrogen production by Clostridium pasteurianum

作者：Yang, Fan[1];Shi, Hongjie[1];Mao, Xingshun[1];Qi, Linfeng[1];Zhu, Minglong[1];Tan, Wensong[1];Zhang, Xu[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：70

起止页码：557

外文期刊名：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

收录：;EI(收录号：20242116114951);WOS:【SCI-EXPANDED(收录号:WOS:001264201700001)】；

基金：This work was supported by National Key Research of China (grant no. 2020YFA0907304) ; The Open Project Funding of State Key Labo- ratory of Bioreactor Engineering of China and National Natural Science Foundation of China (No. 21878083) .

语种：英文

外文关键词：Biohydrogen production; Response surface method; Multi-scale method; C. pasteurianum

摘要：The biohydrogen production in Dark fermentation (DF) could be enhanced by ZnFe 2 O 4 nanomaterials (NPs). The optimum conditions were obtained by a response surface method. Eventually, a biohydrogen production of 2645 mL/L was obtained after 48 h of batch culture, which is 116% higher than control without ZnFe 2 O 4 NPs. The mechanism was investigated by a multi-scale method. In bioreactor scale, ZnFe 2 O 4 NPs could enhance the glucose metabolic ability of C. pasteurianum to promote cell growth and biohydrogen production. The metabolic pathways of biohydrogen production were shifted from the butyric acid-type to the ethanol-type. In cellular scale, ZnFe 2 O 4 NPs could reduce bacterial flagella assembly. In macromolecule scale, the activities of hydrogenase and ethanol dehydrogenase were increased. In genetic scale, the differentially expressed genes mainly involved in biohydrogen production, like those encoded Fe-only hydrogenase, ethanol dehydrogenase, and nitrogenase were up-regulated. The results could provide theoretical and technical supports for the development of commercial biohydrogen process.