文献类型：期刊文献

英文题名：Material design and photo-regulated hydrolytic degradation behavior of tissue engineering scaffolds fabricated via 3D fiber deposition

作者：Yin, Ruixue[1,2];Zhang, Nan[3];Wang, Kemin[3];Long, Hongyu[1];Xing, Tianlong[1];Nie, Jun[4];Zhang, Hongbo[1];Zhang, Wenjun[1,2]

机构：[1]East China Univ Sci & Technol, Complex & Intelligent Res Ctr, Sch Mech & Power Engn, Shanghai 200237, Peoples R China;[2]Univ Saskatchewan, Div Biomed Engn, Saskatoon, SK S7N 5A9, Canada;[3]Changzhou Univ, Sch Mat Sci & Engn, Changzhou 213164, Peoples R China;[4]Beijing Univ Chem Technol, Changzhou Inst Adv Mat, Changzhou 213164, Jiangsu, Peoples R China

年份：2017

卷号：5

期号：2

起止页码：329

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20170303244487);WOS:【SCI-EXPANDED(收录号:WOS:000392418800014)】；

基金：The authors would like to thank the Thousand Talents Plan Project of China and the Fundamental Research Funds for the Central Universities (22A201514029) for the financial support to this work.

语种：英文

外文关键词：Photolysis - Fabrication - Tissue regeneration - Scaffolds (biology) - Deposition - Cell adhesion - Photodegradation

摘要：An ideal behavior of a tissue engineering scaffold is that it degrades and reshapes at a rate that matches the formation of new tissues. However, this ideal situation may not occur as the scaffold often undergoes too slow or too fast degradation. To test the promise of the active control of scaffold degradation, in this work, a photo/water dual-degradable porous scaffold was designed and fabricated using a 3D fiber deposition (3DF) system from a linear biopolymer (named PLANB) that combined the o-nitrobenzyl linkages and hydrolysable ester bone in the polymer chains. The chemical structure, molecular weight and polydispersity of PLANB were characterized by IR, NMR, GPC and MALDI-TOF-MS. The thermal properties of PLANB evaluated by DSC and TGA assays enabled a 3DF printing at the temperature around its melting point without chemical changes. The introduction of a real-time IR (RTIR) technique not only facilitated the determination of photolysis kinetics and quantum yield, but also enabled the capture of intermediate products during the photo-cleavage process of PLANB scaffolds. A minute-scale daily photolysis combined with a continuous hydrolysis process was implemented to test the photo-regulated hydrolytic degradation behavior of PLANB scaffolds in vitro, and the results obtained from both the SEM image and the mass loss profile demonstrated a porous-void microstructure along the strands of scaffolds and an apparent increase of the mass loss amount compared with the control group without photo-irradiation. Furthermore, PLANB scaffolds showed low cytotoxicity to L929 cells and performed well in promoting cell adhesion. It can therefore be concluded that such scaffolds have great potential in offering a diverse range of control over degradation kinetics of tissue engineering scaffolds to be tailored to individual tissue regeneration situations.