文献类型：期刊文献

英文题名：Self-assembly of polyion complex nanocarriers from ammonium glycyrrhizinate and monomethoxy poly(ethyleneglycol)-b-poly(γ-amino-ε-caprolactone)

作者：Zhang, Yan[1];Liu, Jianjing[1];Fu, Yutong[1];Tan, Ke[2];Ye, Zhaoyang[2];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Shanghai Key Lab Adv Polymer Mat,Key Lab Ultrafin, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：1

期号：11

起止页码：1614

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000315169000011)】；

基金：Financial support from the National Natural Science Foundation of China (20804015, 31000424), the Fundamental Research Funds for the Central Universities (WD0913008, WD1014036), Shanghai Key Laboratory Project (08DZ2230500) is gratefully acknowledged. Authors would like to thank Dr Alicia Potuck for her critical reading.

语种：英文

摘要：A novel polyion complex (PIC) drug carrier was developed via electrostatic self-assembly in aqueous media based on our previous copolymer methoxy poly(ethylene glycol)-b-poly(gamma-amino-epsilon-caprolactone) (mPEG-b-PACL) and anti-hepatotoxic drug ammonium glycyrrhizinate (AMG). The formation and the physicochemical properties of the polyion complex nanoparticles in aqueous solution were investigated by various methods. The results demonstrated that AMG was encapsulated successfully and the nanoparticles had a core-shell or tri-layer spherical structure based on the length of the PACL. The AMG/PACL PIC also displayed a high loading capacity and encapsulation efficiency of up to 41.8% and 91.7%, respectively. Furthermore, the nanoparticles enabled a continuous and sustained in vitro AMG release and the release half time was about 3 times that of the free AMG's. Results obtained by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy revealed that AMG loaded nanoparticles demonstrated greater prevention of liver cell apoptosis induced by lipopolysaccharide (LPS). These findings indicated that methoxy poly(ethylene glycol) (mPEG)-b-poly(gamma-amino-epsilon-caprolactone) could be an efficient AMG carrier and could even be used as a delivery template for some other negatively charged water-soluble drugs or gene delivery systems.