文献类型：期刊文献

英文题名：System optimization of an embedding protocol to immobilize cells from Candida bombicola to improve the efficiency of sophorolipids production

作者：Tian, Xiwei[1];Li, Ya[1];Chen, Yang[1];Mohsin, Ali[1];Chu, Ju[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：340

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20213310789507);WOS:【SCI-EXPANDED(收录号:WOS:000696034600010)】；

基金：This work was financially supported by the National Key Research and Development Program (2019YFA0904803), and the Fundamental Research Funds for the Central Universities (JKF01211708 and JKVF1211036). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Immobilization; Sophorolipids; Sodium alginate; Polyvinyl alcohol; Candida bombicola

摘要：This study introduces the implication of immobilization technology in the fermentation process of sophorolipids (SLs) production by Candida bombicola. Firstly, an evaluation system was established for the performance of embedding immobilization and subsequently applied to guide the optimization of operating conditions for sodium alginate immobilization. Correspondingly, the SLs titer increased from 11.4 g/L to 14.6 g/L. Secondly, polyvinyl alcohol was introduced for composite embedding to improve the stability of immobilized beads. Then exogenous addition of 1.5% diatomite further enhanced the fermentation performance of immobilized cells, thereby increasing the SLs titer to 35.9 g/L, which was 2.1 times higher than the original immobilized cells method. Finally, the immobilized cells were tested for three repeated batches of SLs fermentation. Compared to the free cells fermentation, the SLs productivity and substrate conversion rate were increased by 35.5% and 9.1%, respectively. The obtained results showed high potential for application on an industrial scale.