文献类型：期刊文献

英文题名：Adaptive Evolution of Rhodosporidium toruloides with Ultra-centrifugal Fractionation Leads to a High Microbial Lipid Production using Crude Glycerol Feedstock

作者：Wang, Xinru[1];Liu, Qi[1];Zhang, Bin[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：197

期号：12

起止页码：7864

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20254219347823);WOS:【SCI-EXPANDED(收录号:WOS:001594029500001)】；

基金：The authors thank the kind donation of R. toruloides CGMCC 2.1389 by Prof. Zhao ZBK, Dalian Institute of Chemical Physics.

语种：英文

外文关键词：Biodiesel; Crude glycerol; Rhodosporidium toruloides; Microbial lipid; Ultra-centrifugal fractionation

摘要：In situ reintegration of crude glycerol into lipid feedstock can increase the production efficiency and environmental tolerance for the biodiesel industry. In this study, a wild oleaginous yeast Rhodosporidium toruloides CGMCC 2.1389 was selected for its high cell growth and lipid accumulation in crude glycerol. Ultra-centrifugal fractionation was applied in a long-term adaptive evolution, and a stable R. toruloides XR20 strain was finally obtained. The lipid content and titer of the R. toruloides XR20 strain in flask increased by 100.1% and 75.8% compared with the parental strain. Record-breaking lipid titers using crude glycerol by R. toruloides XR20 were achieved by batch and fed-batch fermentation, reaching 24.5 +/- 0.5 g/L and 42.5 +/- 0.8 g/L, respectively. R. toruloides XR20 showed enlarged intracellular space, higher intracellular NADPH, and acetyl-CoA contents compared with the parental strain. The changes in the expression levels of the key genes favored lipid synthesis in R. toruloides XR20. This study constructed a robust high lipid-producing R. toruloides on crude glycerol, contributing to a glycerol-free biodiesel production process.