文献类型：期刊文献

英文题名：Dynamics of base pairs with low stability in RNA by solid-state nuclear magnetic resonance exchange spectroscopy

作者：Zhao, Sha[1,2];Li, Xinming[3];Wen, Ziyang[1];Zou, Mengbing[1];Yu, Ge[2];Liu, Xiangyang[2];Mao, Jiafei[4];Zhang, Lixin[2];Xue, Yi[3];Fu, Riqiang[5,6];Wang, Shenlin[1,2,7]

机构：[1]Peking Univ, Beijing NMR Ctr, Coll Chem & Mol Engn, Beijing 100871, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Tsinghua Univ, Sch Life Sci, Beijing Frontier Res Ctr Biol Struct, Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China;[4]Goethe Univ Frankfurt, Inst Biophys Chem, Max von Laue Str 9, D-60438 Frankfurt, Germany;[5]Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance, Max von Laue Str 9, D-60438 Frankfurt, Germany;[6]Natl High Magnet Field Lab, 1800 East Paul Dirac Dr, Tallahassee, FL 32310 USA;[7]NanKai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

年份：2022

卷号：25

期号：11

外文期刊名：ISCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000882496400006)】；

基金：All of the solid-state NMR experiments were conducted at the Beijing NMR Center and the NMR Facility at the National Center for Protein Sciences at Peking University. The work was supported by the National Key Research and Development Program of the Ministry of Science and Technology of the People?s Republic of China (contract number 2016YFA0501203) , the National Natural Science Foundation of China (22274050, 21874004, 91953104) , the State Key Laboratory of Medicinal Chemical Biology (2020031) , the Fundamental Research Funds for the Central Universities and the Beijing National Laboratory for Molecular Sciences. Dr. R. Fu thanks the support from the National High Magnetic Field Laboratory, which is supported by NSF Cooperative Agreement NSF/DMR-1644779 and the State of Florida. The authors thank Dr. Xiaogang Niu and Dr. Hongwei Li of Peking University for assistance in collecting solution NMR data. The authors thank Dr. Zhijun Liu at the National Center for Protein Science Shanghai for assistance with NMR data collection. Dr S. Wang thank Mr. Weidong Kong from WZ Biosciences Inc (Shandong, China) to provide access to the research facility during the COVID-19 lockdown.

语种：英文

摘要：Base pairs are fundamental building blocks of RNA. The base pairs of low stability are often critical in RNA functions. Here, we develop a solid-state NMR-based water-RNA exchange spectroscopy (WaterREXSY) to characterize RNA in solid. The approach uses different chemical exchange rates between iminos and water to evaluate base pair stability; the less stable ones would exchange more frequently, leading to stronger cross-peaks on WaterREXSY. Applied to the riboA71-adenine complex (the 71nt-aptamer domain of add adenine riboswitch from Vibrio vulnificus), the U47.U51 base pair, which is critical in ligand binding, was found to be less stable than other base pairs. The imino-water exchange rates of U47 at different temperatures are about 500-800 s(-1 ), indeed indicative of low stability. This implies a highly complex and plastic triad involving U47.U51 and that the opening of the U47.U51 base pair may be the early stage of ligand release.