文献类型：期刊文献

英文题名：Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus

作者：Yang, Zhen[1];Zhou, Xiaohui[4,6];Ma, Yue[1,3];Zhou, Mian[1,3];Waldor, Matthew K.[1,5];Zhang, Yuanxing[1,3];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[4]Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA;[5]Harvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA USA;[6]Yangzhou Univ, Jiangsu Key Lab Zoonosis, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

年份：2018

卷号：20

期号：2

起止页码：903

外文期刊名：ENVIRONMENTAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000425019400034)】；

基金：This work was supported by grants from National Natural Science Foundation of China (Nos. 31372560 and 31772891 to QW, 41376128 to YZ, 31772893 to YM), the Ministry of Agriculture of China (CARS-47-G17), the Shanghai Pujiang Program (16PJD018), the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000). XZ was supported by NIH R01AI118943 and National Natural Science Foundation of China (No. 31772741). MKW is supported by Howard Hughes Medical Institute (HHMI) (063101), NIH R37-AI-042347 and China Recruitment Program of High-end Foreign Experts (WQ20143100260).

语种：英文

摘要：Type VI secretion systems (T6SS) are multiprotein secretion machines that can mediate killing of bacterial cells and thereby modify the composition of bacterial communities. The mechanisms that control the production of and secretion of these killing machines are incompletely understood, although quorum sensing (QS) and the PpkA kinase modulate T6SS activity in some organisms. Here we investigated control the T6S in the marine organism Vibrio alginolyticus EPGS, which encodes two T6SS systems (T6SS1 and T6SS2). We found that the organism principally relies on T6SS2 for interbacterial competition. We further carried out a phosphoproteomic screen to identify substrates of the T6SS2-linked PpkA2 kinase. Substrates of PpkA2 encoded within the T6SS2 cluster as well proteins that are apparently not linked to T6SS-related processes were identified. Similar to other organisms, PpkA2 autophosphorylation was critical for T6SS2 function. Notably, phosphorylation of a polypeptide encoded outside of the T6SS2 cluster, VtsR, was critical for T6SS2 expression and function because it augments the expression of luxR, a key regulator of QS that also promotes T6SS2 gene expression. Thus, PpkA2 controls a phosphorylation cascade that mediates a positive regulatory loop entwining T6SS and QS, thereby coordinating these pathways to enhance the competitive fitness of V. alginolyticus.