文献类型：期刊文献

英文题名：Structural elucidation and immunomodulatory activity of a neutral polysaccharide from the Kushui Rose (Rosa setate x Rosa rugosa) waste

作者：Wu, Mengqi[1];Feng, Huafeng[2];Song, Jinxing[1];Chen, Linxiao[1];Xu, Zhizhen[1];Xia, Wei[1];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Bright Dairy & Food Co Ltd, Dairy Res Inst, Shanghai Engn Res Ctr Dairy Biotechnol, State Key Lab Dairy Biotechnol, Shanghai 200436, Peoples R China

年份：2020

卷号：232

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20195307950873);WOS:【SCI-EXPANDED(收录号:WOS:000507231800037)】；

基金：This work was financially supported by the Scientific Fund (19DZ2281400) of Shanghai Engineering Center of Dairy Biotechnology. We would like to thank Shanghai Fangyuan Natural Flavor Co., Ltd. (Shanghai, China) for kindly providing all the Kushui Rose (Rosa setate x Rosa rugosa) waste.

语种：英文

外文关键词：Kushui Rose (Rosa setate x Rosa rugosa) waste; Polysaccharide; Structure; Immunomodulatory

摘要：In this present study, the structure and immunomodulatory activity of a novel polysaccharide (WSRP-1b) from Kushui rose (Rosa setate x Rosa rugosa) waste were investigated. Structure characterization demonstrated that WSRP-1b had a weight-average molecular weight of 1.11 x 10(4) Da and consisted of glucose (42.6 %), mannose (21.4 %), arabinose (9.9 %), xylose (2.2 %), and galactose (23.9 %). Its backbone was composed of 1, 4-linked alpha-Glcp, 1, 4-linked beta-Glcp, and 1, 4-linked beta-Manp, with branches of 1, 4-linked alpha-Glcp and 1, 4-linked beta-Manp substituted at C-6 by 1, 6-linked beta-Galp. The branches mainly contained 1, 5-linked Araf, terminal arabinose and terminal glucose. Bioactivity assays showed that WSRP-1b had immunomodulatory activity by enhancing phagocytosis of macrophages, increasing production of ROS, NO, cytokines (IL-6, TNF-alpha), and activating NF-kappa B signaling pathway. These results suggested that it could be developed as a potential and safe immunomodulatory agent in fields of pharmacological or functional foods.