文献类型：期刊文献

英文题名：Two-step preparation of ginsenoside-Re, Rb1, Rc and Rb 2 from the root of Panax ginseng by high-performance counter-current chromatography

作者：Cheng, Yijun[1,2]; Zhang, Min[1]; Liang, Qionglin[2]; Hu, Ping[3]; Wang, Yiming[2]; Jun, Frank Wu[4]; Luo, Guoan[1,2,5]

机构：[1] Modern Engineering Center for Traditional Chinese Medicine, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; [2] Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China; [3] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; [4] XiangCam TCM Research Centre Ltd, Unit 16, Meditrina, Babraham Research Campus, Cambridge CB22 3AT, United Kingdom; [5] Department of Chemistry, Tsinghua University, Beijing 100084, China

年份：2011

卷号：77

期号：3

起止页码：347

外文期刊名：Separation and Purification Technology

收录：EI(收录号：20110813682984)

语种：英文

外文关键词：Drug products - Dichloromethane - Electrospray ionization - Mass spectrometry - Liquid chromatography - Separation - Acetonitrile - Hexane

摘要：A two-step counter-current chromatography (CCC) method was developed for the preparation of ginsenoside-Re, Rb1, Rc and Rb2 from ginseng with two solvent systems, methylene chloride-methanol-water-isopropanol (6:2:4:3, v/v) and n-hexane-n-butanol-0.1% formic acid (0.7:3:4, v/v). The classical CCC mode was applied in the first separation step while the elution-extrusion counter-current chromatography (EECCC) in the second separation step. The preparation yielded 76.1 mg, 137.0 mg, 58.8 mg and 85.1 mg of ginsenoside-Re, Rb1, Rc and Rb2 with the purity of 97.5%, 94.4%, 91.6% and 92.2%, respectively, from 1440.0 mg of crude sample. The recoveries of these ginsenosides after the two-step separation were 68.7%, 65.6%, 40.1% and 46.6%, respectively. The process throughput of crude sample was 5.14 mg/min while the process efficiencies of ginsenoside-Re, Rb1, Rc and Rb2 were 0.54 mg/mL, 0.49 mg/min, 0.21 mg/min and 0.30 mg/min, respectively. The structures of the targets were characterized by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) and confirmed with the standards. ? 2011 Published by Elsevier B.V. All rights reserved.