文献类型：期刊文献

英文题名：Deciphering the Biosynthesis of TDP-β-L-oleandrose in Avermectin

作者：Tang, Yue[1,2,3];Wang, Min[1,2,3];Qin, Hua[1,2];An, Xiangxiang[1,2];Guo, Zhengyan[1,2];Zhu, Guoliang[4,5];Zhang, Lixin[4,5];Chen, Yihua[1,2,3]

机构：[1]Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China;[2]Chinese Acad Sci, CAS Key Lab Microbial Physiol & Metab Engn, Inst Microbiol, Beijing 100101, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2020

卷号：83

期号：10

起止页码：3199

外文期刊名：JOURNAL OF NATURAL PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000586023100040)】；

基金：We thank Dr. J. Ren, G. Ai, and W. Wang, IM, CAS, for NMR and MS data collection. This work was supported by grants from MOST of China (2018YFA0900700), Center for Ocean Mega-Science, CAS (KEXUE2019GZ05), and China NSFC (31700036, 31720103901). Z.G. is an awardee for the Youth Innovation Promotion Association of CAS (2017124).

语种：英文

摘要：Avermectin (AVM) refers to eight macrolides containing a common L-oleandrosyl disaccharide chain indispensable to their antiparasitic bioactivities. We delineated the biosynthetic pathway of TDP-beta-L-oleandrose (1), the sugar donor of AVM, by characterizing AveBVIII, AveBV, and AveBVII as TDP-sugar 3-ketoreductase, 5-epimerase, and 3-O-methyltransferase, respectively. On the basis of this pathway, we successfully reconstituted the biosynthesis of 1 in Escherichia coli. Our work completes the biosynthetic pathway of AVM and lays a solid foundation for further studies.