文献类型：期刊文献

英文题名：Direct synthesis of Cerium(IV)-Incorporated mesostructured cellular foam for immobilization of penicillin G acylase

作者：Liu, Yi[1,2];Zhang, Haiyan[1];Xu, Xuyang[1];Zhu, Xiaoxiao[1];Liu, Juan[3];Ma, Liang[1];Tian, Chengcheng[1,2]

机构：[1]East China Univ Sci & Technol, Natl Engn Lab Ind Wastewater Treatment, Shanghai 200237, Peoples R China;[2]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：312

外文期刊名：MICROPOROUS AND MESOPOROUS MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000603359900003)】；

基金：We gratefully acknowledge the financial support from the National Natural Science Foundation of China (NSFC, Grant No. 51808225, 21878099), National Key Research and Development Program of China (2019YFA0705800) and the Shanghai Science and technology Innovation Plan (19DZ1208000).

语种：英文

外文关键词：Ce-MCF; Penicillin G acylase; Immobilization; Incorporation; Lewis acid

摘要：Cerium-incorporated mesostructured cellular foams (Ce-MCF) as a novel support has been synthesized successfully for immobilizing penicillin G acylase (PGA) through a Lewis acid-base interaction. The PGA was firmly immobilized on Ce-MCF by interacting with the surface robust Lewis acid sites due to the incorporation of Ce, which is the essence of this immobilization method. The Ce-MCF support was synthesized through the direct hydrothermal synthesis technique with citric acid complexant and categorized by XRD, SAXS, nitrogen sorption, XPS, TEM, and pyridine-IR. The results showed that the Ce-MCF samples had ordered long-range structure, large pore diameter, large specific surface area, pore-volume, narrow pore diameter distribution and strong Lewis acid site. According to the results, the PGA/Ce-MCF has high enzymatic activity and improved operational stability greatly. The PGA/Ce-MCF's initial enzymatic activity remains 10,573 U/g to hydrolyze penicillin G potassium salt, similar with that of PGA/Si-MCF. In addition, the Ce-MCF samples have much higher operational stability than PGA/Si-MCF. Followed by recycling for 10 times, PGA/Ce-MCF preserves 90% of its primary enzymatic activity, which is much higher than 77% of PGA/Si-MCF.