文献类型：期刊文献

英文题名：Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer

作者：Ma, Lin[1,2];Sun, Dongxiao[3];Xiu, Guangli[1];Lazarus, Philip[4];Vachani, Anil[5];Penning, Trevor M.[6];Whitehead, Alexander S.[7];Muscat, Joshua E.[2]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Penn State Coll Med, Dept Publ Hlth Sci, Hershey, PA 17033 USA;[3]Penn State Coll Med, Dept Pharmacol, Mass Spectrometry Core Facil, Hershey, PA 17033 USA;[4]Washington State Univ, Coll Pharm & Pharmaceut Sci, Dept Pharmaceut Sci, Spokane, WA 99210 USA;[5]Univ Penn, Sch Med, Dept Med, Pulm Allergy & Crit Care Div, Philadelphia, PA 19104 USA;[6]Univ Penn, Sch Med, Dept Syst Pharmacol & Translat Therapeut, Philadelphia, PA 19104 USA;[7]Univ Penn, Ctr Pharmacogenet, Sch Med, Dept Pharmacol, Philadelphia, PA 19104 USA

年份：2022

卷号：19

期号：19

外文期刊名：INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH

收录：;WOS:【SSCI(收录号:WOS:000866998900001)，SCI-EXPANDED(收录号:WOS:000866998900001)】；

基金：This study was supported by the China Scholarship Council and by grant PA4100038714 from the Pennsylvania Department of Health (USA). The study was also supported by P30 ES013508 from NIEHS.

语种：英文

外文关键词：oxidative stress; biomarkers; lung cancer; isoprostanes

摘要：Aim: 8-iso-prostaglandin F2 alpha is a biomarker of lipid peroxidation, and one of the most commonly used measures of oxidative stress. It is an established biomarker of lung cancer risk. It is commonly measured by enzyme-linked immunosorbent assay. Given its importance, we developed a stable isotope dilution UPLC-tandem mass spectrometric method for the rapid determination of 8-isoprostane in blood. Methods: We tested the discriminatory capability of the method in 49 lung cancer patients, 55 benign lung nodule patients detected by chest X-ray, and 41 patients with chronic obstructive pulmonary disease (COPD) or asthma. Results: Significant differences were found in mean 8-isoprostane levels between the three groups (p = 0.027), and post-hoc tests found higher levels in the lung cancer patients than in patients with benign nodules (p = 0.032) and COPD/asthma (p = 0.014). The receiving operating characteristic area under the curve (AUC) was 0.69 for differentiating the lung cancer group from the benign nodule group, and 0.7 for differentiating from the COPD/asthma group. Conclusions: The UPLC-MS/MS-based method is an efficient analytical tool for measuring 8-isoprostane plasma concentrations. The results suggest exploring its utility as a marker for early lung cancer screening.