文献类型：期刊文献

英文题名：Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase

作者：Sun, Jian-An[1];Zhang, Liao-Yuan[2];Rao, Ben[1];Shen, Ya-Ling[1];Wei, Dong-Zhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China

年份：2012

卷号：119

起止页码：94

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000307617600015)】；

基金：This work was supported by the Chinese National Program for High Technology and Development (863 Program, No. 2006AA02Z243), Shanghai Leading Academic Discipline Project (No. B505), and State Key Laboratory Special Fund (No. 2060204). We are grateful to Professor Ping Xu (School of Life Science and Technology, Shanghai Jiao Tong University) for giving the nox gene as a present.

语种：英文

外文关键词：Acetoin; 2,3-Butanediol; Serratia marcescens; Cofactor engineering; Fermentation

摘要：Cofactor engineering was employed to enhance production of acetoin by Serratia marcescens H32. 2,3-Butanediol was a major byproduct of acetoin fermentation by S. marcescens H32. In order to decrease 2,3-butanediol formation and achieve a high efficiency of acetoin production, nox gene encoding a water-forming NADH oxidase from Lactobacillus brevis was expressed. Batch fermentations suggested the expression of the NADH oxidase could increase the intracellular NAD(+) concentration (1.5-fold) and NAD(+)/NADH ratio (2.9-fold). Meanwhile, 2,3-butanediol was significantly decreased (52%), and the accumulation of acetoin was enhanced (33%) accordingly. By fed-batch culture of the engineered strain, the final acetoin titer up to 75.2 g/l with the productivity of 1.88 g/(1h) was obtained. To the best of our knowledge, these results were new records on acetoin fermentation ever reported. (C) 2012 Elsevier Ltd. All rights reserved.