文献类型：期刊文献

英文题名：Stereoselective synthesis of chiral δ-lactones via an engineered carbonyl reductase

作者：Wang, Tao[1];Zhang, Xiao-Yan[1];Zheng, Yu-Cong[1];Bai, Yun-Peng[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：57

期号：81

起止页码：10584

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20214211024837);WOS:【SCI-EXPANDED(收录号:WOS:000698787200001)，CCR-EXPANDED(收录号:WOS:000698787200001)】；

语种：英文

外文关键词：Stereoselectivity - Cost effectiveness - Substrates - Esters

摘要：A carbonyl reductase variant, SmCRM5, from Serratia marcescens was obtained through structure-guided directed evolution. The variant showed improved specific activity (U mg(-1)) towards most of the 16 tested substrates and gave high stereoselectivities of up to 99% in the asymmetric synthesis of 13 gamma-/delta-lactones. In particular, SmCRM5 showed a 13.8-fold higher specific activity towards the model substrate, i.e., 5-oxodecanoic acid, and gave (R)-delta-decalactone in 99% ee with a space-time yield (STY) of 301 g L-1 d(-1). The preparative synthesis of six delta-lactones in high yields and with high enantiopurities showed the feasibility of the biocatalytic synthesis of these high-value-added chemicals, providing a cost-effective and green alternative to noble-metal catalysis.