文献类型：期刊文献

英文题名：Genomic and Transcriptomic Evidence Supports Methane Metabolism in Archaeoglobi

作者：Liu, Yi-Fan[1,2,5,6];Chen, Jing[1,2,5];Zaramela, Livia S.[4];Wang, Li-Ying[1,2,5];Mbadinga, Serge Maurice[1,2,5];Hou, Zhao-Wei[7];Wu, Xiao-Lin[7];Gu, Ji-Dong[3];Zengler, Karsten[4];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Hong Kong, Peoples R China;[4]Univ Calif San Diego, Dept Pediat, San Diego, CA 92103 USA;[5]East China Univ Sci & Technol, Engn Res Ctr MEOR, Shanghai, Peoples R China;[6]Shanghai Inst Pollut Control & Ecol Secur, Shanghai, Peoples R China;[7]PetroChina, Daqing Oilfield Co Ltd, Explorat & Dev Res Inst, Daqing, Heilongjiang, Peoples R China

年份：2020

卷号：5

期号：2

外文期刊名：MSYSTEMS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000531077500023)】；

基金：This work was supported by the National Natural Science Foundation of China (grant no. 41807324, 41530318, and 41373070), Fundamental Research Funds for the Central Universities (no. 22221818014 and 50321101917017), and grants from the Research Program of State Key Laboratory of Bioreactor Engineering to Y.-F.L., S.M.M., and B.-Z.M. and from the NSFC/RGC Joint Research Fund (no. 41161160560) to J.-D.G. This study was based in part on work supported by the U.S. Department of Energy (DOE) Office of Science and Office of Biological & Environmental Research (award DE-SC0012586).

语种：英文

外文关键词：horizontal gene transfer; HGT; metatranscriptomics; methyl-coenzyme M reductase complex; MCR complex; oil reservoir; methanogens

摘要：Euryarchaeal lineages have been believed to have a methanogenic last common ancestor. However, members of euryarchaeal Archaeoglobi have long been considered nonmethanogenic and their evolutionary history remains elusive. Here, three high- quality metagenomic-assembled genomes (MAGs) retrieved from high-temperature oil reservoir and hot springs, together with three newly assembled Archaeoglobi MAGs from previously reported hot spring metagenomes, are demonstrated to represent a novel genus of Archaeoglobaceae, "Candidatus Methanomixophus." All "Ca. Methanomixophus" MAGs encode an M methyltransferase (MTR) complex and a traditional type of methyl-coenzyme M reductase (MCR) complex, which is different from the divergent MCR complexes found in "Ca. Polytropus marinifundus." In addition, "Ca. Methanomixophus dualitatem" MAGs preserve the genomic capacity for dissimilatory sulfate reduction. Comparative phylogenetic analysis supports a laterally transferred origin for an MCR complex and vertical heritage of the MTR complex in this lineage. Metatranscriptomic analysis revealed concomitant in situ activity of hydrogen-dependent methylotrophic methanogenesis and heterotrophic fermentation within populations of "Ca. Methanomixo-phus hydrogenotrophicum" in a high-temperature oil reservoir. IMPORTANCE Current understanding of the diversity, biology, and ecology of Archaea is very limited, especially considering how few of the known phyla have been cultured or genomically explored. The reconstruction of "Ca. Methanomixophus" MAGs not only expands the known range of metabolic versatility of the members of Archaeoglobi but also suggests that the phylogenetic distribution of MCR and MTR complexes is even wider than previously anticipated.