文献类型：期刊文献

英文题名：Quantitative determination of rhamnolipid using HPLC-UV through carboxyl labeling

作者：Zhou, Jing[1,2];Miao, Si-Jia[1,2];Yang, Shi-Zhong[1,2,3,4];Liu, Jin-Feng[1,2,3];Gang, Hong-Ze[1,2,3];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[3]Minist Educ, Engn Res Ctr Microbial Enhanced Oil Recovery, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Meilong 130, Shanghai 200237, Peoples R China

年份：2023

卷号：70

期号：6

起止页码：1806

外文期刊名：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录：;EI(收录号：20232414222730);WOS:【SCI-EXPANDED(收录号:WOS:001000767000001)】；

基金：National Key Research and Development Program of China, Grant/Award Number: 2022YFC2105200; Fundamental Research Funds for the Central Universities, Grant/Award Number: JKJ01231714; Natural Science Foundation of Shanghai, Grant/Award Number: 21ZR1417400; National Natural Science Foundation of China, Grant/Award Numbers: 42061134011, 42173076, 52074129

语种：英文

外文关键词：biosurfactant; derivatization; Pseudomonas aeruginosa; quantification; rhamnolipid

摘要：Rhamnolipid, as a low-toxic, biodegradable and environmentally friendly biosurfactant, has broad application prospects in many industries. However, the quantitative determination of rhamnolipid is still a challenging task. Here, a new sensitive method for the quantitative analysis of rhamnolipid based on a simple derivatization reaction was developed. In this study, 3-[3 '-(l-rhamnopyranosyloxy) decanoyloxy] decanoic acid (Rha-C10-C10) and 3-[3 '-(2 '-O-alpha-l-rhamnopyranosyloxy) decanoyloxy] decanoic acid (Rha-Rha-C10-C10) were utilized as the representative rhamnolipids. Liquid chromatography-mass spectrometry and high-performance liquid chromatography-ultra violet results showed that these two compounds were successfully labeled with 1 N1-(4-nitrophenyl)-1,2-ethylenediamine. There was an excellent linear relationship between rhamnolipid concentration and peak area of labeled rhamnolipid. The detection limits of the Rha-C10-C10 and Rha-Rha-C10-C10 were 0.018 mg/L (36 nmol/L) and 0.014 mg/L (22 nmol/L), respectively. The established amidation method was suitable for the accurate analysis of rhamnolipids in the biotechnological process. The method had good reproducibility with the relative standard deviation of 0.96% and 0.79%, respectively, and sufficient accuracy with a recovery of 96%-100%. This method was applied to quantitative analysis of 10 rhamnolipid homologs metabolized by Pseudomonas aeruginosa LJ-8. The single labeling method was used for the quantitative analysis of multiple components, which provided an effective method for the quality evaluation of other glycolipids with carboxyl groups.