文献类型：期刊文献

英文题名：A β-D-galactose-guided fluorescent probe for selectively bioimaging endogenous formaldehyde in living HepG-2 cells

作者：Zhou, Langping[1];Cui, Jingjing[1];Yu, Zhihao[1];Zou, Dongxuan[1];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：332

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20210409825665);WOS:【SCI-EXPANDED(收录号:WOS:000618774600003)】；

基金：This work was financially supported by NSFC (21576085 and 21974045).

语种：英文

外文关键词：beta-D-galactose; Formaldehyde; HepG-2 cells; Fluorescence detection; Bioimaging

摘要：A fluorescent probe NFP-G, with beta-D-galactose as the targeted unit and hydrazine as the recognition group for formaldehyde (FA), was designed and synthesized for specifically detecting endogenous FA in HepG-2 cells via asialoglycopmtein receptor-mediated endocytosis. The experimental results show that probes NFP-G/NFP-A exhibited highly fluorescent responses toward FA, which could be ascribed to the restriction of the photoinduced electron transfer (PET) process by the formation of hydrazone. Good selectivity and competition of NFP-G toward FA over potentially interfering species ensure it an appropriate candidate for FA detection. The sensing mechanism of the probes toward FA was studied by theoretical calculation, high performance liquid chromatography and mass spectrum. The confocal bioimaging results reveal that NFP-G could selectively visualize the endogenous FA in asialoglycoprotein receptor overexpressed HepG-2 cells over other cells. Pretreated with beta-galactosidase made both asialoglycopmtein receptor positive and negative cells display strong green fluorescence, revealing beta-D-galactose is essential in targeting HepG-2 cells.