文献类型：期刊文献

英文题名：Development of a method for trace level determination of antibiotics in drinking water sources by high performance liquid chromatography-tandem mass spectrometry

作者：Cheng, Wenhuan[1,2];Jiang, Lei[3];Lu, Ning[3];Ma, Lei[1];Sun, Xiaoyan[1,2];Luo, Yi[4];Lin, Kuangfei[2];Cui, Changzheng[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[3]Natl Engn Res Ctr Urban Water Resources, Shanghai 200082, Peoples R China;[4]Naikai Univ, Tianjin Key Lab Environm Remediat & Pollut Contro, Key Lab Pollut Proc & Environm Criteria, Coll Environm Sci & Engn,Minist Educ, Tianjin 300071, Peoples R China

年份：2015

卷号：7

期号：5

起止页码：1777

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20151000608226);WOS:【SCI-EXPANDED(收录号:WOS:000350443800017)】；

基金：This research was supported by the National Water Pollution Control and Management Technology Major Projects (no. 2012ZX07403-002) and the National Environmental Protection Public Welfare Science and Technology Research Program of China (no. 201309031) and the Shanghai Rising-Star Program, China (no. 14QB1400800).

语种：英文

外文关键词：Extraction - Phase separation - Mass spectrometry - High performance liquid chromatography - Potable water - Trace analysis - Water quality

摘要：The presence of antibiotics in drinking water sources is worthy of concern regarding their potentially harmful effects on drinking water quality. In this study, a sensitive and reliable method was developed for the detection of 14 antibiotics in drinking water sources based on solid phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The primary parameters for the SPE procedure, including different SPE cartridges, pH value of the sample, extraction volume and washing conditions, were optimized to extract the analytes efficiently in a single step with improved recoveries. Chromatographic separation conditions and MS/MS parameters in multiple reaction monitoring (MRM) mode were optimized to improve the sensitivity and specificity of the method. The optimized method provided acceptable recoveries ranging from 60.5% to 103.3%. The validation study indicated that the method detection limits varied from 0.001 to 2.160 ng L-1 and the method quantification limits varied from 0.003 to 6.740 ng L-1. The precision of the method, expressed as relative standard deviation (RSD), ranged from 0.1% to 2.6% and from 0.3% to 3.8% for inter- and intra-day analyses, respectively. Assessment of matrix effects exhibited partial signal suppression from 1.2% to 28.7% for most analytes, but it indicated signal enhancement for tetracycline (15.2%) and oxytracycline (12.6%). The method was successfully applied to the determination of trace levels of antibiotics in drinking water sources in East China. Up to 13 antibiotics were detected at concentrations ranging from 0.16 to 147.05 ng L-1, and the primary antibiotic residues belonged to the groups of fluoroquinolones and tetracyclines.