文献类型：期刊文献

英文题名：Multicopy integrants of crt genes and co-expression of AMP deaminase improve lycopene production in Yarrowia lipolytica

作者：Zhang, Xin-Kai[1];Nie, Ming-Yue[1];Chen, Jun[1];Wei, Liu-Jing[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：289

起止页码：46

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20184706129143);WOS:【SCI-EXPANDED(收录号:WOS:000453380200007)】；

基金：This study was financially supported by National Natural Science Foundation of China (21576089, 21776081).

语种：英文

外文关键词：Lycopene; Yarrowia lipolytica; Multicopy integration; AMP deaminase overexpression; Fermenter cultivation

摘要：Lycopene has been broadly studied in recent decades due to its health benefits including cancer prevention, anti-atherogenic and anti-obesity effects, and modulation of the immune system. To obtain efficient synthesis of lycopene, extensive researches have been conducted in various microbial cells, including Yarrowia lipolytica, to heterologously produce lycopene using various genetic and metabolic engineering methods. In this study, the effects of copy numbers of lycopene synthesis genes, a variety of key central metabolic genes (especially AMP deaminase-encoding gene AMPD), and 5-L fermenter cultivation on lycopene production in Y. lipolytica were investigated and the engineered strains with significantly enhanced lycopene content (46-60 mg/g DCW) were achieved. It is therefore possible to make use of the obtained strains to meet the industrial demand of lycopene production on the basis of further genetic and process optimization.