文献类型：期刊文献

英文题名：Protein-Triggered Reassembly of Quinocyanine Nanosheets for Intraoperative NIR-II Cholangiography

作者：Zhang, Yutao[1,2];Wu, Menglan[1,2];Li, Wennan[1,2];Liu, Zhiyong[3];Liu, Caiqi[1,2];Wu, Yue[4];Yan, Chenxu[1,2];Xu, Yisheng[4];Zhu, Wei-Hong[1,2];Guo, Zhiqian[1,2]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Feringa Nobel Prize Scientist, Key Lab Adv Mat,Inst Fine Chem,Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China;[3]Fudan Univ, Inst Forens Sci, Inst Biomed Sci, Shanghai 200032, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Green Chem Engn & Ind Catalysis, State Key Lab Chem Engn, Shanghai 200237, Peoples R China

年份：2026

卷号：65

期号：5

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20255119735072);WOS:【SCI-EXPANDED(收录号:WOS:001638380900001)】；

基金：This work was supported by National Key Research and Development Program (2023YFA1802000), NSFC/China(22225805, 22308101, 32394001, 22378126, and 32121005),Shanghai Science and Technology Innovation Action Plan(No. 23J21901600), Natural Science Foundation of Shanghai(23ZR1416600). Thanks to Yifan He of Xinhua Hospital,Shanghai Jiao Tong University School of Medicine, for hishelp in the in vivo experiments.

语种：英文

外文关键词：Activatable imaging; Cholangiography; Fluorescent probe; NIR-II fluorescence; Quinocyanine dye

摘要：Intraoperative near-infrared II (NIR-II) fluorescence imaging offers high-contrast, real-time visualization during surgery, yet the development of activatable probes remains a formidable challenge because most tissue-specific biomarkers, such as receptor proteins, are chemically inert intrinsic. Here, we present a protein-triggered reassembly strategy in which planar NIR-II dyes pre-assemble into pi-pi stacked aggregates with quenched fluorescence that reorganize into highly emissive dye-protein complexes upon target binding, thereby achieving activatable intraoperative imaging. Compared with indocyanine green (ICG), a clinically approved "always-on" cholangiography agent, quinocyanine green (QCG) was developed as a bile specifical activated NIR-II probe through stepwise molecular engineering. Native PAGE, protein sequencing, and TEM analyses all revealed that a hemoglobin subunit in bile drives the transformation of the initial QCG nanosheets into uniform homogeneous spherical protein-dye complexes, yielding a remarkable 310-fold fluorescence enhancement at 1011 nm. Taking such advantage, for the first time, we have clearly and directly visualized intrahepatic bile duct in vivo. Moreover, QCG afforded high-contrast, precise intraoperative cholangiography, allowing detailed biliary tree mapping, stricture identification, bile leakage detection, and dual-color hepatobiliary imaging. This in situ reassembly strategy establishes a generalizable molecular design principle for activatable NIR-II probes and expands the toolbox for precision fluorescence-guided surgery.