文献类型：期刊文献

英文题名：Simultaneous determination of β-lactam antibiotics and β-lactamase inhibitors in bovine milk by ultra performance liquid chromatography-tandem mass spectrometry

作者：Li, Nasi[1,2];Feng, Feng[2];Yang, Bingcheng[1];Jiang, Pingping[2];Chu, Xiaogang[2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing 100123, Peoples R China

年份：2014

卷号：945

起止页码：110

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20135117100959);WOS:【SCI-EXPANDED(收录号:WOS:000330147700015)】；

基金：The research was sponsored by Research Special Funds for Public Welfare Project (No. 2012104002).

语种：英文

外文关键词：beta-Lactam antibiotics; beta-Lactamase inhibitors; Ultra-high performance liquid; chromatography; Tandem mass spectrometry; Bovine milk

摘要：An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method has been developed for the simultaneous determination of four beta-lactam antibiotics (amoxicillin, ampicillin, cefotaxime, and cefoperazone) and two P-lactamase inhibitors (tazobactam, sulbactam) in bovine milk. The analytes were extracted with water from bovine milk and purified with Oasis HLB solid phase extraction (SPE) cartridges. The analytes were determined in less than 3 min by UPLC-MS/MS in positive and negative electrospray ionization (ESI) modes, separately. The method was linear over the range of 1-100 mu g/L for tazobactam, sulbactam, ampicillin, and cefoperazone, and 2-100 mu g/L for amoxicillin and cefotaxime. The recoveries for all six analytes in bovine milk ranged from 82.5 to 98.3%. The limits of detection and the limits of quantitation were 0.1-0.2 mu g/L and 0.3-0.5 mu g/L, respectively. The intra- and inter-day precisions were less than 6% for each compound. (C) 2013 Elsevier B.V. All rights reserved.