文献类型：期刊文献

英文题名：Construction of a genetically engineered microorganism for phenanthrene biodegradation

作者：Zhou, Ying[1];Wei, Jiashi[1];Shao, Naimin[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, Newworld Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：53

期号：2

起止页码：188

外文期刊名：JOURNAL OF BASIC MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000314924500013)】；

基金：The plasmid was kindly provided by Dr. Qiyao Wang. This work was supported by grants from the Ministry of Science and Technology, P.R. China (project nos. 2002AA2Z345A and 2004AA2Z3801).

语种：英文

外文关键词：Genetically engineered microorganisms; pRK415; Biodegradation; Polycyclic aromatic hydrocarbons; Phenanthrene

摘要：The bacterium Pseudomonas sp. CGMCC2953, isolated from oil-polluted soil, was used as a recipient for a biodegradative gene encoding catechol 2,3-dioxygenase (C23O), which was successfully cloned into the plasmid pK4 derived from pRK415 with a broad host range. The apparent phenanthrene biodegradation parameters of the recombinant microorganism (Pseudomonas sp. CGMCC2953-pK) were determined and compared with those of the wild type. As the key enzyme of phenanthrene degradation, C23O, could be expressed constitutively in the recombinant strain, Pseudomonas sp. CGMCC2953-pK showed an increased ability to degrade phenanthrene. The excessive production of C23O in Pseudomonas sp. CGMCC2953-pK could serve as an effective approach to construct genetically engineered microorganisms for the bioremediation of environmental contaminations.