文献类型：期刊文献

英文题名：Exploration of glyoxals' level changes in endoplasmic reticulum during ferroptosis by a photocaged fluorescent probe

作者：Xing, Wanjin[1,2];Zhang, Yirong[1,2];Ma, Huijuan[1,2];Shen, Shuran[1,2];Wang, Sinan[3,4];Xu, Huan[5];Wang, Wei[6,7];Lou, Kaiyan[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]ShanghaiTech Univ, Sch Biomed Engn, 393 Huaxia Rd, Shanghai 201210, Peoples R China;[4]ShanghaiTech Univ, State Key Lab Adv Med Mat & Devices, 393 Huaxia Rd, Shanghai 201210, Peoples R China;[5]Anhui Univ Sci & Technol, Sch Publ Hlth, Hefei 231131, Anhui, Peoples R China;[6]Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA;[7]Univ Arizona, Inst BIO5, Tucson, AZ 85721 USA

年份：2025

卷号：423

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20244117178662);WOS:【SCI-EXPANDED(收录号:WOS:001335317500001)】；

基金：This work was supported by Shanghai Natural Science Fund (Grant No. 20ZR1414700) , Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission) , National Natural Science Foundation of China (Grant No. 42177417) , and the Undergraduate Innovation and Entrepreneurship Training Program, ECUST (Grant No. X202310251130) .

语种：英文

外文关键词：Photocaged fluorescent probe; Ferroptosis; Glyoxals; Endoplasmic reticulum; Dicarbonyl stress

摘要：Both ferroptosis and elevated glyoxals (e.g. methylglyoxal and glyoxal), are associated with endoplasmic reticulum stress, but their correlations, in particular, how level of glyoxals changes in endoplasmic reticulum during ferroptosis remains unclear. In this work, a new photoactivatable fluorescent probe Photo-ER-GOS was developed to study level changes of glyoxals in endoplasmic reticulum during ferroptosis via in situ photodecaging and fluorescence detection, avoiding potential interference from glyoxals in cytosol. The probe contains a photolabile nitrobenzyloxymethyl group functioned both as the reactivity blocking group and the fluorescence quenching group, which linked to the guanidino group of NAP-DCP-3, , the active endoplasmic reticulum-targeting fluorescent probe for glyoxals previously developed in our lab. It was found that the level of glyoxals in endoplasmic reticulum increases significantly during erastin-induced ferroptosis in RAW 264.7 cells via comparison of relative fluorescence intensity increases. The endoplasmic reticulum glyoxal level increase was also confirmed by the Ultra Performance Liquid Chromatography-Mass Spectrum studies. In contrast, no significant change of level of glyoxals in endoplasmic reticulum was found in staurosporine-induced apoptosis, suggesting that upregulated glyoxals in endoplasmic reticulum may be a previously overlooked characteristic of ferroptosis. Erastin-induced level increase of glyoxals was also found in zebrafish. The caged probe Photo-ER-GOS thus provides a useful chemical tool to study GOS levels changes in endoplasmic reticulum.