文献类型：期刊文献

英文题名：Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil

作者：Li, Jianhua[1];Yang, Yiming[1];Chu, Ju[1];Huang, Mingzhi[1];Li, Liang[1];Zhang, Xiaochun[2];Wang, Yonghong[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanxi Weiqida Pharmaceut Co Ltd, Shanxi 037000, Peoples R China

年份：2010

卷号：33

期号：9

起止页码：1119

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20104313321892);WOS:【SCI-EXPANDED(收录号:WOS:000283080500013)】；

基金：This work was financially supported by a grant from the National High Technology Research and Development Program of China (863 Program), No. 2006AA020302, the Major State Basic Research Development Program of China (973 Program), No. 2007CB714303, National Key Technology Research and Development Program, No. 2008BAI63B01, 2007BAI26B02, and Shanghai Leading Academic Discipline Project, No. B505.

语种：英文

外文关键词：Metabolic flux analysis; Acremonium chrysogenum; Fed-batch cultivation; Cephalosporin C

摘要：A metabolic network was constructed for the Acremonium chrysogenum cultivation fed with soybean oil. Metabolic flux analysis indicated that the shift from exponential growth to rapid cephalosporin C (CPC) formation was accompanied by 1.63- and 5-fold carbon flux enlargement in TCA cycle and glyoxylate by-pass, respectively. The flux via pentose phosphate pathway branch was little affected during the rapid CPC formation period; the contributory explanation was that 35.6% of NADPH was consumed in the dissimilation of fatty acids. Estimation of NADPH, ATP generation, and consumption demonstrated that, with soybean oil as carbon source in rapid CPC formation phase, the NADPH consumed in fatty acid catabolism was fourfold greater than that used in the CPC biosynthesis-relevant part; simultaneously, more than 90% energy spent was not directly related to the CPC formation. Therefore, the improvement of CPC production yield through optimization of the NADPH, ATP generation, and consumption was put forward.