文献类型：期刊文献

中文题名：溶氧水平和磷酸盐对庆大霉素合成与分泌的影响

英文题名：Influence of dissolved oxygen and phosphate on the synthesis and secretion of gentamicin

作者：张达力[1];储炬[1];李友荣[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：27

期号：3

起止页码：145

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 39870 0 1 8

语种：中文

中文关键词：庆大霉素;溶氧;磷酸盐;生物合成;分泌;次级代谢

外文关键词：Gentamicin; Dissolved oxygen; Phosphate; Synthesis; Secretion

摘要：研究了溶氧与磷酸盐对庆大霉素合成与分泌的影响。在摇瓶实验中不同的装液量对庆大霉素的效价与分泌率都有显著的影响 ,在发酵罐上控制溶氧水平在 40 %左右有利于庆大霉素合成与分泌。添加一定浓度的磷酸盐对菌体生长有利 ,但却抑制庆大霉素的合成与分泌 ,当磷酸盐浓度高于 0 .0 5 %时 ,次级代谢基本被抑制。
Effect of dissolved oxygen and phosphate on the synthesis and secretion of gentmicin (GM) were studied In flask fermentation, the influence of different cultrue volume on the synthesis and secretion of GM was significant. Results showed that the synthesis and secretion of GM increased when DO was 40%. Low concentration of phosphate can facilitate growth of mycelium, but the synthesis and secretion of GM were restrained when concentration of phosphate was hligher than 0.05%.