文献类型：期刊文献

英文题名：Simultaneous Surface-Enhanced Raman Spectroscopy Detection of Multiplexed MicroRNA Biomarkers

作者：Zhou, Wen[1];Tian, Ya-Fei[1];Yin, Bin-Cheng[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang 832000, Peoples R China

年份：2017

卷号：89

期号：11

起止页码：6121

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20172503808969);WOS:【SCI-EXPANDED(收录号:WOS:000403036800065)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grants 21335003, 21675052, 21575089), the Science Fund for Creative Research Groups (Grant 21421004), Programme of Introducing Talents of Discipline to Universities (Grant B16017), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Biomarkers - Diseases - Raman scattering - Substrates - Synthesis (chemical) - Diagnosis - Surface scattering - Raman spectroscopy - RNA - Cancer cells - Gold nanoparticles

摘要：Simultaneous detection of cancer biomarkers holds great promise for the early diagnosis of cancer. In the present work, an ultrasensitive and reliable surface-enhanced Raman scattering (SERS) sensor has been developed for simultaneous detection of multiple liver cancer related microRNA (miRNA) biomarkers. We first proposed a novel strategy for the synthesis of nanogap-based SERS nanotags by modifying gold nanoparticles (AuNPs) with thiolated DNA and nonfluorescent small encoding molecules. We also explored a simple approach to a green synthesis of hollow silver microspheres (Ag-HMSs) with bacteria as templates. On the basis of the sandwich hybridization assay, probe DNA-conjugated SERS nanotags used as SERS nanoprobes and capture DNA-conjugated Ag-HMSs used as capture substrates were developed for the detection of target miRNA with a detection limit of 10 EM. Multiplexing capability for simultaneous detection of the three liver cancer related miRNAs with the high sensitivity and specificity was demonstrated using the proposed SERS sensor. Furthermore, the practicability of the SERS sensor was supported by the successful determination of target miRNA in cancer cells. The experimental results indicated that the proposed strategy holds significant potential for multiplex detection of cancer biomarkers and offers the opportunity for future applications in clinical diagnosis.