文献类型：期刊文献

英文题名：Functional Living Materials for Virus Disinfection Based on Engineered Bacillus subtilis Biofilm by Displaying Viral Affinity Peptides and Expressing Protease

作者：Zhu, Xiaojuan[1];Chen, Jianshu[1];Luo, Jian[2];Liu, Xue[3];Xu, Yan[1];Chen, Lin[1];Dong, Hao[4];Zhang, Lingfan[5];Yang, Lin[6];Jiang, Nin[1];Huang, Xumeng[1];Mohsin, Ali[1];Yi, Xiaoping[1];Wang, Yonghong[1];Zhuang, Yingping[1];Zhang, Xuehong[7];Zhang, Ke[2];Zhang, Lixin[1];Zhong, Chao[8];Huang, Jiaofang[1,6]

机构：[1]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Inst Biol Prod Co Ltd, Shanghai, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Immun & Infect, Shanghai 200031, Peoples R China;[4]Ocean Univ China, Coll Food Sci & Engn, State Key Lab Marine Food Proc & Safety Control, Qingdao 266404, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[6]Jiangxi Normal Univ JXNU, Coll Life Sci, Nanchang 330022, Peoples R China;[7]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[8]Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

年份：2024

卷号：34

期号：44

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20243316879224);WOS:【SCI-EXPANDED(收录号:WOS:001289836300001)】；

基金：This work was supported by the National Key Research and Development Program of China (Grant No. 2020YFA0908900), the Natural Science Foundation of Shanghai (Grant No. 22ZR1416000), the Open Funding Project of State Key Laboratory of Microbial Metabolism (Grant No. MMLKF24-01) for J.F.H., and the National Science Fund for Distinguished Young Scholars (grant number 32125023) for C.Z. The authors also thank the Research Center of Analysis and Test (ECUST) for help on data and characterization. The authors thank Professor Fener Chen (JXNU) and Weishan Wang (Institute of Microbiology, CAS) for their help and comments.

语种：英文

外文关键词：Bacillus subtilis biofilm; engineered living materials; SARS-CoV-2; virus disinfection

摘要：Global pandemics caused by pathogenic viruses have highlighted the need to develop effective and sustainable materials to defend against these viruses. However, most commercial viral disinfection materials rely on precious metals, which are toxic. In this study, an engineered living material based on Bacillus subtilis biofilms is presented that can effectively remove Influenza A virus and SARS-CoV-2 from water. First, B. subtilis biofilm is used to display the H1N1 and H5N1 specific binding peptide C40 and SARS-CoV-2 specific binding peptide SBP1; thus, the biofilm materials has outstanding efficacy against H1N1, H5N1, and SARS-CoV-2 pseudovirus from water. In addition to capturing the virus, these biofilm materials can also inactivate the virus by secreting alkaline protease AprE, which effectively degrades the viral proteins at temperatures of 4, 16, 25, and 37 degrees C. Furthermore, to constrain bacterial growth and prevent the leakage of genetically modified organisms, a suicide genetic circuit using the quorum-sensing system ComQXPA to control Lytc expression is constructed, leading to bacterial lysis. Overall, this study demonstrates a novel, effective, and sustainably engineered living material for preventing viral transmission. This material provides a safeguard for public health and promotes environmentally friendly governance.