文献类型：期刊文献

英文题名：L-GLUTAMATE BIOSENSOR USING A NOVEL L-GLUTAMATE OXIDASE AND ITS APPLICATION TO FLOW-INJECTION ANALYSIS SYSTEM

作者：Ye, Bang-Ce[1]; Li, Qing-Shan[1]; Li, You-Rong[1]; Li, Xiao-Bo[1]; Yu, Jun-Tang[1]

机构：[1]E CHINA UNIV SCI & TECHNOL,BIOCHEM ENGN RES INST,SHANGHAI 200237,PEOPLES R CHINA

年份：1995

卷号：42

期号：1

起止页码：45

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：1996223030951);WOS:【SCI-EXPANDED(收录号:WOS:A1995RR95200005)】；

语种：英文

外文关键词：L-GLUTAMATE OXIDASE; L-GLUTAMATE BIOSENSOR; L-GLUTAMATE DETERMINATION; PLATINIZED PLATINUM ELECTRODE; FLOW INJECTION ANALYSIS

摘要：Streptomyces P-106, isolated from soil samples, produced extracellular L-glutamate oxidase in liquid culture. About 10 units enzyme activity per ml medium could be reached after 48-60 h incubation at 28 degrees C. A micro-enzyme electrode was prepared by cross linking the purified novel L-glutamate oxidase, produced in our lab, with glutaraldehyde on an aminopropyl-platinized-platinum wire (phi 0.5 mm). It was used for the determination of L-glutamate in a now injection analysis system with good performance: accuracy (CV = 0.4%), fast response (< 40 s) and stability(> 30 d). The system showed linear response to the L-glutamate concentration, ranging from 3.0 mg l(-1) to 300 mg l(-1) (approx. 0.02 mol l(-1) to 2.0 mmol l(-1)). The system was applied to determine the concentration of L-glutamic acid during synthesis. Good correlations were achieved between results obtained with the system and with Warburg method.