文献类型：期刊文献

英文题名：Enhanced production of fumigaclavine C by ultrasound stimulation in a two-stage culture of Aspergillus fumigatus CY018

作者：Yao, Ling-Yun[1];Zhu, Yi-Xiang[1];Jiao, Rui-Hua[2];Lu, Yan-Hua[1];Tan, Ren-Xiang[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Eng, Shanghai 200237, Peoples R China;[2]Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Inst Funct Biomol, Nanjing 210008, Jiangsu, Peoples R China

年份：2014

卷号：159

起止页码：112

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20141217488820);WOS:【SCI-EXPANDED(收录号:WOS:000335393500016)】；

基金：The authors are grateful for funding from the National High Technology Research and Development Program of China (2011AA090702 and 2013AA092901), the Fundamental Research Funds for the Central Universities, the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204) as well as Shanghai Leading Academic Discipline Project (B505). The technical assistance of Ying-Bo Li (Institute of Process Engineering, Chinese Academy of Sciences) is gratefully acknowledged.

语种：英文

外文关键词：Conidiation; Fumigaclavine C; Morphology; Two-stage culture; Low-intensity ultrasound

摘要：Stimulation by physical means including ultrasound is important to cell morphology and the product yield. In this work, the effect of ultrasound on the production of fumigaclavine C (FC), a conidiation-associated alkaloid with strong anti-inflammatory activity, was investigated in a newly developed two-stage culture of Aspergillus fumigatus CY018. The optimum ultrasonication conditions consisted of exposing cultures (at 12 h of growth phase) to 10-min repeated irradiation (4 times) with a 24-h interval at the fixed power (500 W). Under this condition, FC production reached 118.09 mg/L, which was 89% higher than the control and much higher than previous reported values. Morphological analysis demonstrated that mycelia morphology from ultrasonication was in the form smaller and looser pellets as compared to that of the control. In addition, conidia that is closely related to FC biosynthesis were significantly increased after ultrasound stimulation, with 3 folds of that from the control. (c) 2014 Elsevier Ltd. All rights reserved.