文献类型：期刊文献

英文题名：A novel paradigm for the high-efficient production of phycocyanin from Galdieria sulphuraria

作者：Wan, Minxi[1];Wang, Zhenyang[1];Zhang, Zhen[1];Wang, Jun[2];Li, Shulan[3];Yu, Anquan[2];Li, Yuanguang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Yunnan Shangrila Zeyuan Ltd Co Algal Hlth Technol, Diqing 674412, Peoples R China;[3]Jiaxing Zeyuan Bioprod Co Ltd, Jiaxing 314006, Peoples R China

年份：2016

卷号：218

起止页码：272

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20162702560805);WOS:【SCI-EXPANDED(收录号:WOS:000384710500037)】；

基金：This research was funded by National High Technology Research and Development Program of China (2015AA020602), the Fundamental Research Funds for the Central Universities (222201414024). The authors declared that they have no conflicts of interest to this work.

语种：英文

外文关键词：Sequential Heterotrophy-Dilution-Photoinduction; Galdieria sulphuraria; Phycocyanin

摘要：A novel cultivation strategy called "Sequential Heterotrophy-Dilution-Photoinduction" was successfully applied in the cultivation of Galdieria sulphuraria for efficient production of phycocyanin (PC). Algae cells were firstly cultivated heterotrophically to achieve high cell density. The maximal dry cell weight of 17.8 g L-1 and maximal biomass productivity of 103.1 mg L-1 h(-1) were obtained. Then, a dilution step was applied to obtain a suitable cell concentration and finally these cells were transferred to high light condition for phycocyanin accumulation. During the photoinduction step, cells could accumulate phycocyanin up to 13.88% of dry cell weight in a cultivation period of 8 days. By this technology, total PC productivity far surpassed those reported in all literatures of Galdieria sulphuraria, and was 147-fold and 12-fold of those in photoautotrophic and heterotrophic technologies, respectively. Therefore, this strategy provides a promising approach for high-efficient phycocyanin production from Galdieria sulphuraria to meet its mass cultivation and commercialization application. (C) 2016 Elsevier Ltd. All rights reserved.