文献类型：期刊文献

英文题名：Engineering oleaginous yeast Yarrowia lipolytica for enhanced limonene production from xylose and lignocellulosic hydrolysate

作者：Yao, Feng[1];Liu, Shun-Cheng[1];Wang, Dan-Ni[1];Liu, Zhi-Jie[2];Hua, Qiang[1,3];Wei, Liu-Jing[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Hubei Univ Technol, Minist Educ, Key Lab Fermentat Engn, Wuhan 430068, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：20

期号：6

外文期刊名：FEMS YEAST RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000582304400005)】；

基金：We thank Prof. Jie Bao for the generous gift of detoxified lignocellulose hydrolysate. This research was financially supported by the National Natural Science Foundation of China (21776081, 21576089) and the Natural Science Foundation of Shanghai (18ZR1410000).

语种：英文

外文关键词：limonene; xylose; Yarrowia lipolytica; lignocellulosic hydrolysate

摘要：Limonene, a valuable cyclic monoterpene, has been broadly studied in recent decades due to its wide application in the food, cosmetics and pharmaceutical industries. Engineering of the yeast Yarrowia lipolytica for fermentation of renewable biomass lignocellulosic hydrolysate may reduce the cost and improve the economics of bioconversion for the production of limonene. The aim of this study was to engineer Y. lipolytica to produce limonene from xylose and low-cost lignocellulosic feedstock. The heterologous genes XR and XDH and native gene XK encoding xylose assimilation enzymes, along with the heterologous genes tNDPS1 and tLS encoding orthogonal limonene biosynthetic enzymes, were introduced into the Po1f strain to facilitate xylose fermentation to limonene. The initially developed strain produced 0.44 mg/L of limonene in 72 h with 20 g/L of xylose. Overexpression of genes from the mevalonate pathway, including HMG1 and ERG12, significantly increased limonene production from xylose to similar to 9.00 mg/L in 72 h. Furthermore, limonene production peaked at 20.57 mg/L with 50% hydrolysate after 72 h when detoxified lignocellulosic hydrolysate was used. This study is the first to report limonene production by yeast from lignocellulosic feedstock, and these results indicate the initial steps toward economical and sustainable production of isoprenoids from renewable biomass by engineered Y. lipolytica.