文献类型：期刊文献

英文题名：Biohydrogen Production by a Novel Strain Petroclostridium Sp. X23 Isolated from the Production Water of Oil Reservoirs

作者：Fang, Bo[1,2]; Liu, Yi-Fan[1,2]; Pan, Xu-Jie[1]; Zhou, Lei[1,2]; Yang, Shi-Zhong[1,2]; Gu, Ji-Dong[3]; Mu, Bo-Zhong[1,2]

机构：[1] State Key Laboratory of Bioreactor Engineering, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] Engineering Research Center of Microbial Enhanced Oil Recovery, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [3] Environmental Engineering, Guangdong Technion Israel Institute of Technology, 241 Daxue Road, Guangdong, Shantou, 515063, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20240009198)

语种：英文

外文关键词：Alkalinity - Glucose - Hydrogen production - Petroleum reservoir engineering - Petroleum reservoirs - RNA - Volatile fatty acids

摘要：The subsurface-sourced natural hydrogen is a great potential but undervalued clean energy. To explore the role of biohydrogen from microorganisms in the deep subsurface oil-reservoir environment, we report a biohydrogen-producing bacterial strain isolated from a long-term methanogenic crude oil-degrading enrichment culture of the production water from an oil reservoir. Based on the phylogenetic analysis of the 16S rRNA sequence, strain X23 was identified as the second member of the genus Petroclostridium. The impact of various temperature, pH, and glucose concentrations on hydrogen production by Petroclostridium sp. X23 was investigated. Under the conditions of 37°C, initial pH 8.0 and glucose concentration of 4g/L, the strain X23 achieved its maximum hydrogen yield at 1.82±0.12 mol H2/mol glucose. In comparison to its close relative Petroclostridium xylanilyticum SK-Y3, the strain X23 exhibited higher hydrogen production and hydrogen yield. The volatile fatty acids of acetate and formate were generated during glucose fermentation. Hdr complex catalyzed the oxidation of formate and proton reduction and produced hydrogen. These findings indicate that Petroclostridium sp. X23 is a potential candidate for dark fermentative hydrogen production under alkaline and mesophilic conditions. ? 2023, The Authors. All rights reserved.