文献类型：期刊文献

英文题名：Four Flavonoid Compounds from Phyllostachys edulis Leaf Extract Retard the Digestion of Starch and Its Working Mechanisms

作者：Yang, Jun-Peng[1,2];He, Hao[1,2];Lu, Yan-Hua[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2014

卷号：62

期号：31

起止页码：7760

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20151600747580);WOS:【SCI-EXPANDED(收录号:WOS:000340080100015)】；

基金：This work was supported by the Fundamental Research Funds for the Central Universities and partially supported by the Shanghai Leading Academic Discipline Project (Grant B505) and the National Special Fund for State Key Laboratory of Bioreactor Engineering (Grant 2060204).

语种：英文

外文关键词：bamboo leaf; orientin; isoorientin; vitexin; isovitexin; diabetes; alpha-amylase; starch

摘要：Bamboo leaf extract as a food additive has been used for preventing the oxidation of food. In the present study, we investigated the influence of Phyllostachys edulis leaf extract on starch digestion. Orientin, isoorientin, vitexin, and isovitexin were determined as its a-amylase inhibitory constituents. An inhibitory kinetics experiment demonstrated that they competitively inhibit a-amylase with K.; values of respectively 152.6, 11.5, 569.6, and 75.8 mu g/mL. Molecular docking showed the four flavones can interact with the active site of a-amylase, and their inhibitory activity was greatly influenced by the glucoside linking position and 3'-hydroxyl. Moreover, the results of starch iodine complex spectroscopy, X-ray diffraction, and scanning electron microscopy indicated that P. edulis flavonoids retard the digestion of starch not only through interaction with digestive enzymes, but also through interaction with starch. Thus, P. edulis leaf extract can be potentially used as a starch-based food additive for adjusting postprandial hyperglycemia.