文献类型：期刊文献

英文题名：Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine

作者：Zhang, Fang[1]; Sun, Xiangming[1]; Yi, Xiaoping[1]; Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：51

期号：1

起止页码：21

外文期刊名：CYTOTECHNOLOGY

收录：;EI(收录号：20242716575031);WOS:【SCI-EXPANDED(收录号:WOS:000241376600003)】；

语种：英文

外文关键词：animal cell culture; cell metabolism; glutamine; glutamine synthetase; recombinant CHO cell

摘要：To elucidate the metabolic characteristics of recombinant CHO cells expressing glutamine synthetase (GS) in the medium with or without glutamine, the concentrations of extra-and intracellular metabolites and the activities of key metabolic enzymes involved in glutamine metabolism pathway were determined. In the absence of glutamine, glutamate was utilized for glutamine synthesis, while the production of ammonia was greatly decreased. In addition, the expression of recombinant protein was increased by 18%. Interestingly, the intracellular glutamine maintained almost constant, independent of the presence of glutamine or not. Activities of glutamate-oxaloacetate aminotransferase (GOT), glutamate-pyruvate aminotransferase (GPT), and glutamate dehydrogenase (GDH) increased in the absence of glutamine. On the other hand, intracellular isocitrate and the activities of its downstream isocitrate dehydrogenase in the TCA cycle increased also. In combination with these two factors, a 8-fold increase in the intracellular a-ketoglutarate was observed in the culture of CHO-GS cells in the medium without glutamine.