文献类型：期刊文献

中文题名：甲醇利用型细菌发酵生产吡咯喹啉醌的培养条件

英文题名：Study on the Production of Pyrroloquinoline Quinone by Using Methanol-utilizing Bacteria

作者：尹芳[1];陆兵[1];陈国豪[1];徐殿胜[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室生物工程系,上海200237

年份：2004

卷号：30

期号：2

起止页码：227

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：吡咯喹啉醌;生丝微菌;甲醇;Fe^2+;发酵

外文关键词：PQQ; Hyphomicrobium; methanol; Fe^(2+); fermentation

摘要：以甲醇为唯一碳源,对生丝微菌TH205进行培养,研究了甲醇流加速度、Fe2+浓度对细胞生长及吡咯喹啉醌(PQQ)表达量的影响。结果表明:培养基中添加浓度为7μg/mL的Fe2+,并控制甲醇的流加速度使培养基中甲醇浓度为1mL/L时,细胞的生长情况较好,且此时PQQ的发酵产量为26.6μg/mL。
In fermentation experiments with feeding Hyphomicrobium TH205 using methanol as single carbon source the effects of feeding of methanol and adding of Fe^(2+) to the basal medium on the production of PQQ were investigated. Results showed that when 7 μg/mL of Fe^(2+) was added, and the residual concentration of methanol was controlled at 1 mL/L in broth by controlling during the cultivation, the production of PQQ reached 26.6 μg/mL.