文献类型：期刊文献

英文题名：Physiological and transcriptome analysis elucidates the metabolic mechanism of versatile Porphyridium purpureum under nitrogen deprivation for exopolysaccharides accumulation

作者：Ji, Liang[1];Li, Shaohua[1];Chen, Cheng[1];Jin, Haojie[2];Wu, Haizhen[1,3];Fan, Jianhua[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China;[3]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China

年份：2021

卷号：8

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513079278);WOS:【SCI-EXPANDED(收录号:WOS:000685308900001)】；

基金：This work was sponsored by National Key Research and Development Project of China 2019YFA0906300 and 2020YFA0907304, Natural Science Foundation of Shandong Province ZR2019ZD17, National Natural Science Foundation of China 31872608, Natural Science Foundation of Shanghai 21ZR1416400 and 18ZR1410100, Shanghai Pujiang Program 18PJD008, and Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Porphyridium purpureum; Nitrogen deprivation; Transcriptome analysis; Exopolysaccharides; Polyunsaturated fatty acids; Phycoerythrin

摘要：Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibited the growth of P. purpureum and resulted in yellowing of the cells and thickening of the extracellular viscousness sheath. Under nitrogen stress, the contents of total lipids and exopolysaccharides in P. purpureum were increased by 65.2% and 188.0%, respectively. We demonstrate that the immediate response of P. purpureum to nitrogen deficiency is mediated by carbon flow to polysaccharide synthesis, while the synthesis of lipids is enhanced as a permanent energy storage substance at the later stage. Based on transcriptome annotation information, we elucidate the synthesis pathway of polysaccharides from P. purpureum from the perspective of glycosyl-donor interconversion, and demonstrate that the n-6 pathway is the main synthesis pathway of polyunsaturated fatty acids. This study not only provides a production strategy for polysaccharides and fatty acids by single-celled marine red algae P. purpureum, but also provides targets for further genetic modification.