文献类型：期刊文献

英文题名：The effects of surface bioactivity and sustained-release of genistein from a mesoporous magnesium-calcium-silicate/PK composite stimulating cell responses in vitro, and promoting osteogenesis and enhancing osseointegration in vivo

作者：Cai, Liang[1];Zhang, Jue[1];Qian, Jun[1];Li, Quan[2];Li, Hong[3];Yan, Yonggang[3];Wei, Shicheng[4];Wei, Jie[1];Su, Jiacan[2]

机构：[1]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Second Mil Med Univ, Changhai Hosp, Dept Orthopaed Trauma, Shanghai 200433, Peoples R China;[3]Sichuan Univ, Coll Phys Sci & Technol, Chengdu 610041, Sichuan, Peoples R China;[4]Peking Univ, Sch & Hosp Stomatol, Lab Interdisciplinary Studies, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China

年份：2018

卷号：6

期号：4

起止页码：842

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20181404976113);WOS:【SCI-EXPANDED(收录号:WOS:000428550100015)】；

基金：The grants were from the National Key R&D Program (2016YFC1102100), the National Natural Science Foundation of China (81771990, 51502340, 51772194 and 81572194), the Major International Joint Research Project between China and Korea (81461148033) and the Opening Project of Shanghai Key Laboratory of Orthopaedic Implants.

语种：英文

外文关键词：Surface roughness - Bioactivity - Computerized tomography - Calcium silicate - Cell proliferation - Magnesium compounds - Silicates

摘要：The surface of a mesoporous magnesium-calcium-silicate (m-MCS)/polyetheretherketone (PK) composite (MPC) was modified by sand blasting, and genistein (GS) was loaded inside the nanopores of the m-MCS on the modified MPC (MPCm) surface. The results showed that compared with MPC, the surface roughness and hydrophilcity of MPCm obviously improved with more m-MCS exposed on its surface. Moreover, no obvious differences in surface roughness and hydrophilcity were found between MPCm and GS loaded MPCm (MPCm-Ge), and both of them possessed an improved apatite mineralization ability in simulated body fluid solution (SBF) compared with MPC, indicating excellent surface bioactivity. Moreover, the MPCm obviously stimulated the adhesion, proliferation, differentiation and gene expressions of MC3T3-E1 cells compared with MPC, and the sustained-release of GS from the MPCm-Ge surface further significantly promoted the cell proliferation, differentiation and gene expression. According to the Micro-CT, histological and SEM analysis, the results demonstrated that the MPCm obviously improved osteogenesis and enhanced osseointegration in vivo compared with MPC, and the release of GS from the MPCm-Ge surface further significantly improved osteogenesis and enhanced osseointegration. In summary, the significant promotion of cell responses in vitro, and the improvements of osteogenesis and the enhancement of osseointegration in vivo were attributed to the effects of surface bioactivity and GS sustained-release from the MPCm-Ge surface. Therefore, MPCm-Ge would be a potential candidate for orthopedic and dental applications.