文献类型：期刊文献

中文题名：基于时间常数与CFD相结合的反应器放大方法

英文题名：Scale-up of bioreactor based on time constant analysis and computational fluid dynamics

作者：李超[1];夏建业[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：2019

卷号：47

期号：10

起止页码：63

中文期刊名：化学工程

外文期刊名：Chemical Engineering(China)

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金面上项目(21776082)

语种：中文

中文关键词：生物反应器;时间常数;计算流体力学;放大方法

外文关键词：bioreactor;time constant;computational fluid dynamics;scale-up

摘要：为解决生物发酵过程放大问题,文中提出了一种基于时间常数分析与计算流体力学(CFD)相结合的生物反应器放大方法。首先根据大肠杆菌发酵过程生理代谢数据,对诱导前后两阶段氧消耗和底物消耗时间常数进行了计算。通过菌体"消耗型"时间常数与设备的"供给型"时间常数的对比分析发现,诱导前为供氧条件限制,而诱导后为混合条件限制。在菌体生长期需保证氧传递时间常数tmt<4.2 s,即kLa>0.236 s^-1;而在诱导期需保证混合时间tm<36 s。据此,对工业规模20 t生物反应器进行了理性设计,并通过CFD方法对设计方案进行验证。结果表明:设计的反应器kLa大于0.236 s^-1,且混合时间小于36 s,氧传递和混合性能均达到设计要求,能满足诱导型大肠杆菌高密度发酵过程的需求。
To address the issues in scale-up of bioreactors, a scale-up strategy based on time constant analysis and computational fluid dynamics(CFD) was proposed. First, time constants about oxygen and substrate consumptions in growth phase and inducing phase were determined according to the physiological metabolism analysis of E.coli fermentation. By the comparison of time constants related to metabolism and those related to bioreactor performance, oxygen transfer was suggested to be the key factor in the growth phase, but mixing in the inducing phase. Specifically, the time constant for oxygen transfer tmt <4.2 s, or kLa>0.236 s^-1 should be ensured in the growth phase, and the mixing time tm<36 s should be ensured in the inducing phase. Based on the findings, a 20 m3 bioreactor was rationally designed, and its performances were verified by CFD methods. The results show that the bioreactor possesses kLa>0.236 s^-1 and tm<36 s, meeting the requirements of high-cell-density fermentation of E. coli.